曼陀罗茎段愈伤组织诱导和再生植株的研究  

Research on Inducing Callus from Datura metel L.Stem and Plantlet Regeneration

作　　者：黄静[1] 赵琦[1] 赵玉锦[2] 李楠[1] 张世煌[2] 

机构地区：[1]首都师范大学生命科学学院,北京100037 [2]中国农业科学院作物科学研究所玉米研究中心,北京100081

出　　处：《生物技术通报》2007年第5期179-183,共5页Biotechnology Bulletin

摘　　要：本试验以曼陀罗茎段为外植体,在附加不同植物激素组合的培养基中对愈伤组织的诱导和植株再生进行研究。结果表明:采用修改的MS培养基(除去甘氨酸,维生素B1含量增加至0.5mg/L,pH5.5)附加2mg/L2,4-D可由曼陀罗茎段诱导大量胚性愈伤组织;愈伤组织继代选用0.5mg/L2,4-D为宜;不定芽的诱导采用MS培养基(20g蔗糖,8g琼脂,0.1g水解干酪素)+6-BA(0.5mg/L);幼苗进一步转接至1/2MS+IBA(0.2mg/L)生根培养基中,可完成曼陀罗茎段愈伤组织诱导和再生植株的组织培养过程。The callus induction and plantlet regeneration were researched on the different plant hormone combination media with Datura metel L.stem as explants.The results showed as below：Datura metel L.callus were induced on the modified MS containing vitamin B1 0.5mg/L and 2,4-D 2mg/L without glycin,pH5.5.The Datura metel L.callus were subcultured on the modified MS containing 2,4-D 0.5mg/L.The plantlet regeneration were performed on the MS medium containing sucrose 20g,hydrolyze casein 0.1g and 0.5mg/L 6-BA.1/2MS medium containing 0.2mg/L IBA was suitable for root growth.

关 键 词：曼陀罗 愈伤组织 诱导 组织培养 

分 类 号：S567.219[农业科学—中草药栽培] Q943.1[农业科学—作物学]