西洋参悬浮细胞发酵工艺研究  

Studies on Fermentation of Suspension Cells of Panax quinquefolium

作　　者：张小兵[1] 闫静辉[1] 李亚璞[1] 吴萌[1] 李春生[1] 陈英珠[1] 程华[1] 

机构地区：[1]河北省生物研究所,石家庄050081

出　　处：《生物技术通报》2007年第5期188-193,共6页Biotechnology Bulletin

基　　金：河北省科技攻关招标项目(02245512D)

摘　　要：探讨了西洋参悬浮细胞分步培养与稀土、D-半乳糖和甘露醇等诱导子对悬浮细胞生长及皂甙产量的影响。发现继代4d后换液一次再继续培养获得的培养物,在皂甙产率和糖利用率等方面优于连续培养;D-半乳糖作为诱导子,对悬浮培养的西洋参细胞生长、皂甙产率及皂甙的分泌等方面都有非常明显的促进作用。The effects of a feeding culture and several elicitors on cell growth and saponin production in Panax quinquefolium suspension cell culture were investigated in the study.The results revealed that the feeding culture was better for the production of saponin than the continuous culture,and D-galactose as elicitor obviously stimulated the cell growth and saponin secretion.

关 键 词：西洋参悬浮细胞 人参皂甙 分步培养 诱导子 

分 类 号：Q943.1[生物学—植物学] S567.53[农业科学—中草药栽培]