慢性浅表性胃炎脾胃湿热证与胃黏膜水通道蛋白3、4基因表达的相关性研究  

Study on Correlation between Aquaporin 3,4 Gene Expression in Gastric Mucosa and Severity of Pi-Wei Damp-Heat Syndrome in Patients with Chronic Superficial Gastritis

作　　者：梅武轩[1] 劳绍贤[2] 周正[2] 黄烈平[2] 胡斌[3] 

机构地区：[1]湖北咸宁学院医学院内科教研室,湖北437100 [2]广州中医药大学 [3]中山大学达安基因诊断中心

出　　处：《中国中西医结合杂志》2007年第10期891-893,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金面上项目(No.30271572)

摘　　要：目的探讨慢性浅表性胃炎脾胃湿热证不同程度与胃黏膜水通道蛋白3、4(AQP3、AQP4)基因表达的相关性。方法胃镜下取胃体上部黏膜,液氮罐保存,荧光定量PCR法检测胃黏膜AQP3、AQP4的基因表达。结果脾胃湿热中度组、重度组AQP3、AQP4基因表达水平均高于对照组和脾胃湿热轻度组(P<0.05,P<0.01);脾胃湿热重度组AQP3高于脾胃湿热中度组(P<0.05)。结论脾胃湿热程度不同,胃黏膜AQP3、AQP4基因表达不同,脾胃湿热程度与胃黏膜AQP3、AQP4基因表达相关,它随着湿热程度的加重而增加。Objective To explore the relationship between aquaporin 3,4 （AQP3, AQP4） gene expression in gastric mucosa and severity of Pi-Wei damp-heat syndrome （PWDHS） in patients with chronic superficial gastritis （CSG）. Methods Gastric mucosa taken from the upper part of gastric corpus was collected under gastro-scope and preserved in liquid nitrogen. The gene expression of AQP3 and AQP4 was determined quantitatively by fluorescent PCR. Results The gene expression of AQP3 and AQP4 in patients with PWDHS of moderate and severe degree was higher than that in those of mild degree and in healthy persons respectively （P 〈0.05 and P 〈 0.01 ） ; and the gene expression of AQP3 in patients with PWDHS of severe degree was higher than that in those of moderate degree （P 〈 0.05）. Conclusion The gene expression of AQP3 and AQP4 in gastric mucosa was correlative with the severity of PWDHS in patients with chronic superficial gastritis, the severer the syndrome, the higher the gene expression.

关 键 词：慢性浅表性胃炎 水通道蛋白 基因表达 

分 类 号：R259[医药卫生—中西医结合]