祛瘀生肌中药对大鼠正常创面肉芽组织中Ⅰ型胶原和基质金属蛋白酶表达的影响  

Influence of Stasis-removing and Muscle-regenerating Chinese Hebal Medicine on the Expression of Collagen TypeⅠand Matrix Metalloproteinase-1 in Granulation Tissue of Ordinary Wound

作　　者：肖秀丽[1] 李斌[1] 王振宜[1] 李福伦[1] 范斌[1] 徐文彬[1] 金炜[1] 王一飞[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院皮肤科,上海200437

出　　处：《中国中西医结合杂志》2007年第10期909-911,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.39900195);上海市重点学科建设项目资助项目(No.T0304)

摘　　要：目的探讨祛瘀生肌中药对大鼠正常创面肉芽组织影响的差异。方法以120只大鼠皮肤全层缺损创面为模型,采用免疫组化和图像分析相结合的方法,观察生肌化瘀方及其拆方生肌方和化瘀方对创面修复不同时期肉芽组织中基质金属蛋白酶(matrix metalloproteinase-1,MMP-1)和Ⅰ型胶原变化的影响。结果正常组Ⅰ型胶原表达水平明显延迟,MMP-1水平在创伤后3～11天里呈阶梯式增高;生肌方早期可以促进创面Ⅰ型胶原及MMP-1分泌,从创伤后3～11天一直维持在较高水平;化瘀方组MMP-1表达在前7天保持较高水平,与生肌方组比较差异有显著性(P<0.05),创伤后11天降低,显著低于正常组和生肌方组(P<0.05);生肌化瘀方小剂量组促进Ⅰ型胶原分泌有两个高峰时期,即创伤后7天和15天,与正常组比较差异有显著性(P<0.05);生肌化瘀方大剂量组在11天时MMP-1表达显著降低,明显低于正常组(P<0.05)。结论祛瘀生肌中药对实验性创面新生肉芽组织中Ⅰ型胶原及MMP-1表达的影响,可能是在创面愈合的过程中,通过抑制MMP-1的分泌,从而使创面Ⅰ型胶原的分泌增加,起到促进创面愈合的作用。Objective To understand the effects of Chinese herbal medicine for stasis-removing （SR） and muscle-regenerating （MR）, in different constitutions, on granulation tissue of ordinary wound. Methods Shengji Huayu recipe, a common used Chinese recipe for SR ＆ MR （SR-MR）, and its disassembled recipe, Shengji recipe （MR） and Huayu recipe （SR）, were applied resectively on the full-layer skin injured wound in 120 rats to observe their effects on collagen type I （C I ） and matrix metalloproteinase-1 （MMP-1） in granulation tissue of wound at various stages of healing with immunohistochemistry technique and image-analysis system. Results The expression of C I of the normal group was obviously delayed and the level of MMP-1 increased in a ladder type from day 3 to day 11. Shengji group could promote the secretion of C I and MMP-1 at the early stage and keep a high level from day 3 to day 11. The expression of MMP-1 of Huayu group main-tained a high level on the first 7 days with significant difference as compared with the Shengji group （P 〈 0.05）, and reduced from day 11 showing significant difference when compared with the normal group and Shengji group （all P〈0.05）. Shengji Huayu low dose group has two periods of peak time in promoting the secretion of C I on day 7 and 15, and showed significant difference as compared with the normal group （P 〈 0.05） ; Shengji Huayu high dose group could reduce the MMP-1 on day 11 and obviously lower when compared with the normal group （P〈0.05）. Conclusion The mechanism of Chinese herbal medicine for SR-MR in promoting wound healing was probably through inhibiting the secretion of MMP-1 to increase the C I content in granulation tissue of wound.

关 键 词：祛瘀生肌中药 创面Ⅰ型胶原 基质金属蛋白酶 

分 类 号：R285.5[医药卫生—中药学]