丙型肝炎病毒结构基因转基因小鼠的建立  

TRANSGENIC MICE FROM FERTILIZED EGGS MICROINJECTED WITH HEPATITIS C VIRUS STRUCTURAL GENES.

作　　者：谭文杰[1,2] 丛郁[1,2] 李光三 毕胜利[1,2] 杜淼[1,2] 詹美云 

机构地区：[1]中国预防医学科学院病毒学研究所 [2]中国科学院发育生物学研究所

出　　处：《病毒学报》1997年第1期19-23,共5页Chinese Journal of Virology

基　　金：中国预防医学科学院病毒学研究所科学基金

摘　　要：为探讨丙型肝炎病毒（ＨＣＶ）结构基因在ＨＣＶ感染中的致病性，构建了中国丙型肝炎病毒５ＵＴＲ区与结构基因区（Ｃ＋Ｅ１＋Ｅ２）的表达质粒，并通过显微注射法将其接种入小鼠受精卵内制备转基因小鼠。共注射受精卵４１０枚，存活３１２枚，植入后产仔６０只；转基因鼠尾部组织ＰＣＲ法ＤＮＡ检测证明有靶基因的整合；转基因小鼠的肝、肾、脾、心、肺、小肠、血中均有靶基因的转录，而在脑组织中无转录。３只Ｇｏ代整合小鼠经与正常ＩＣＲ小鼠配种，获Ｇ１代小鼠３２只，其中１３只有ＨＣＶ结构基因的整合。这一结果表明，ＨＣＶ结构基因转基因小鼠动物模型已建成。To investigate the role of hepatitis C virus structural proteins in the pathogenesis of hepatitis C infection,we produced transgenic mice carrying HCV structural genes(C,E1 and E2)by microinjecting the gene that contained the CMV promoter and HCV structural genes.The microinjected fertilized eggs were 410,the survived eggs were 312,and 60 transgenic mice were born.Transgenic mice were identified by subjecting tail DNA to amplification by polymerase chain reaction using three sets of oligonucleotides as special primers.We also proved the presence of transcripts of the HCV structural genes in the liver,kidney,spleen,heart,lung,intestine tissues and blood but not in the brain by RT-PCR.These results demonstrate transgenic mice model for HCV structural genes has been established.

关 键 词：丙型肝炎病毒 结构基因 转基因小鼠 

分 类 号：R373.21[医药卫生—病原生物学]