棉铃虫核型多角体病毒多角体蛋白基因的克隆和核苷酸序列分析  

CLONING AND SEQUENCING OF HELICOVERPA ARMIGERA NUCLEAR POLYHEDROSIS VIRUS POLYHEDRIN GENE

作　　者：王根[1,2,3] 张传溪[1,2,3] 贡成良 金伟[1,2,3] 吴祥甫 

机构地区：[1]中国科学院上海生物化学研究所 [2]浙江农业大学 [3]苏州大学

出　　处：《病毒学报》1997年第1期83-87,共5页Chinese Journal of Virology

摘　　要：棉铃虫核型多角体病毒多角体蛋白基因的克隆和核苷酸序列分析王根张传溪贡成良金伟吴祥甫（中国科学院上海生物化学研究所，上海２０００３１）关键词棉铃虫，核型多角体病毒，多角体蛋白基因，核苷酸序列，同源性棉铃虫Ｈｅｌｉｃｏｖｅｒｐａ（Ｈｅｌｉｏｔｈｉ...The polyhedrin gene (Ph) of Helicoverpa (Heliothis) armigera,a singlenucleocapsid nuclear polyhedrosis virus (HaSNPV) Hubei isolate located within a 4.2kb SalⅠ fragment,was cloned using BmNPV Ph gene as α- 32 P-labeled probe and Southern hybridization method.1701 nucleotides (nt) of the genome containing the Ph was sequenced,and an open reading frame (ORF) of 738nt encoded a protein of 246 amino acids with a MW of 29kD was predicted.With canonical ATAAG promoter motif,a conserved 14-nt sequence (Rohrmann box) AAAATAAGTATTTT associated with transcriptional start sites in other polyhedrin genes was identified at -48 to -52 nt upstream from the start codon.A putative polyadenylation signal AATAAA,was found at 258 nt downstream of the polypeptide translational stop codon.Comparison of the nucleotide sequence and deduced amino acid of HaSNPV Ph with other NPV polyhedrins and granulosis virus (GV) granulins showed a high degree of homology with 20 other NPVs,particularly closely related to that of HzSNPV (97%),and most distantly related to that of Neodiprion sertifer SNPV(44.5%).

关 键 词：昆虫病毒 棉铃虫 核型多角体病毒 蛋白基因 

分 类 号：Q965.8[生物学—昆虫学]