一株斑点热立克次体的病原学研究  

STUDIES ON THE FEATURES OF THE STRAIN OF SPOTTED FEVER GROUP RICKETTSIAE

作　　者：陈香蕊[1] 胥照平[1] 陈万荣[1] 李申龙 张永国[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《微生物学通报》1997年第1期31-34,共4页Microbiology China

摘　　要：自内蒙古自治区的草原革蜱（D．nuttalli）中分离到一株斑点热立克次体。用微量酶标染色方法进行血清学鉴定表明，系西伯利亚（Rickettsiaesibirica种血清型。G＋Cmol％含量为32.4％。对其核酸进行聚合酶链扩增／限制性核酸内切酶消化（PCR／RFLP）分析DNA长度多态性，分别引用立氏立克次体蛋白抗原基因Rr190序列作为扩增引物Rr190．70p～602n和Rr190．4442p～5664n，扩增后分别用Pst1和Rsal限制性核酸内切酶消化。其电泳图谱证明，该分离株的DNA多态图谱与R．sibirica完全一致。上述结果可确证该分离株为西伯利亚斑点热立克次体种，称内85011株即NM-8501。The features of the NM8501 strain of Spotted Fever Group Rickettsiae (SFGR) fromticks in HalLaer city, Inner Mongolia were analysed ,The serotype of NM8501 was the same withR. sibirica (strain 246 and Jinghe); It's DAN base composition, G+C mol%, was 32.4% that wasidentical with those of other members of SFGR. The genetic type of NM8501 were no differmtfrom 246 and Jinghe strains of R. sibirica specie of SFGR in PCR /RFLP polyacrylamideelectrophologram of Rr190.70p ～ 602n (Pst1) and Rr190.4442p～ 5664n (Ras1)． The results aboverevealed that NM8501 strain was thougt as sibirica space of SFGR.

关 键 词：立克次体 斑点热立克次体 病原学 

分 类 号：R376[医药卫生—病原生物学]