秦岭野生雌性川金丝猴粪便睾酮水平与邀配频次的季节性变化  

Seasonal changes of fecal testosterone levels and solicitation frequency in female Sichuan snub-nosed monkeys Rhinopithecus roxellana in the Qinling Mountains

作　　者：高云芳[1] 王慧平[1] 李保国[1] 

机构地区：[1]西北大学生命科学学院,西安710069

出　　处：《动物学报》2007年第5期783-790,共8页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金(No.30370202);陕西省自然科学基金(No.DH98273)资助~~

摘　　要：为研究野生雌性川金丝猴(Rhinopithecus roxellana)不同季节体内睾酮水平与性行为频次的变化及二者的关系,我们以秦岭一野生川金丝猴种群为研究对象,通过长时间连续跟踪观察,完成了该种群中所有87只金丝猴的个体识别。用无损伤取样法采集了三只成年雌性川金丝猴不同季节的粪样,对粪样中的睾酮进行抽提测定,同时对采样个体的性行为进行记录。结果显示:1)成年雌猴在交配盛期与非交配盛期,粪样中睾酮水平均呈明显的周期性波动,交配盛期时睾酮水平的变化周期为24.56 d±4.07 d,非交配盛期时为43.67 d±2.89 d,显著长于交配盛期;2)成年雌猴在交配盛期的粪样睾酮水平为2.85 ng±1.12 ng/g,邀配频次为0.98±1.04次/d,非交配盛期时睾酮水平为1.71 ng±0.77 ng/g,邀配频次为0.28±0.53次/d,与交配盛期相比,非交配盛期的睾酮水平和邀配频次均明显降低;3)交配盛期各成年雌猴的粪样睾酮水平与邀配频次均有较显著的正相关关系(相关系数分别为0.631、0.683和0.659),而在非交配盛期这种相关关系并不存在(相关系数分别为0.091、0.493和0.205)。本研究表明,成年雌性川金丝猴体内睾酮水平、睾酮水平变化周期及邀配行为频次均具有明显的季节性差异,这种季节性差异可能是川金丝猴行季节性繁殖的内在原因和外在表现之一。本研究亦表明交配盛期雌猴的性行为频次在一定程度上可能受体内睾酮水平的控制,而在非交配盛期这种控制作用较弱。In order to study seasonal changes in testosterone levels and frequency of solicitation in wild female Sichuan snub-nosed monkeys （ Rhinopithecus roxellana ）, one group of wild golden monkeys in the Qinling Mountains was selected as the research target. By continuous tracking and observation, individual identification had been achieved for all 87 individuals in this group. Applying non-invasive sampling methods, we collected fecal samples and data on sexual behavior from three adult female monkeys. Fecal T （Testosterone, T） levels were measured by radioimmunoassay （RIA）, after extracting the hormone from fecal samples. The results showed that fecal T levels in wild adult female monkeys displayed obvious cyclic fluctuations during dominant and non- dominant mating periods. In the dominant mating period, the mean length of cycles was 24.56 d ± 4.07 d and significantly shorter than that during the non-dominant mating period （43.67 d ± 2.89 d）. The mean fecal T level was 2.85 ng ± 1.12 ng/g and the sexual solicitation frequency was 0.98 ± 1.04 cases/d in the dominant mating period. Both of these measures were significantly higher than in the non-dominant mating period, （ 1.71 ng ± 0.77 ng/g and O. 28 ± 0.53 cases/d, respectively）. In the dominant mating period, frequencies of sexual behavior of every target individual significantly correlated with its fecal T level, （the correlation coefficients were 0.631, 0.683 and 0.659, respectively）. However, these correlations did not occur during non-dominant mating period （the correlation coefficients were 0.091, 0.493 and 0.205, respectively）. This research suggests that the T levels, the T level cycle length and sexual solicitation frequency all undergo seasonal changes. Seasonal changes may be the intrinsic reasons and extrinsic exhibitions of seasonal reproduction in golden monkeys. In addition, this research also suggests that for adult female monkeys, frequencies of solicitation may be regulated by T levels during dominant mating period

关 键 词：川金丝猴 无损伤采样法 睾酮 周期变化 邀配行为 季节性繁殖 

分 类 号：Q958[生物学—动物学]