百合花粉母细胞核骨架的超微结构观察  

ULTRASTRUCTURAL OBSERVATIONS ON NUCLEAR MATRIX IN THE POLLEN MOTHER CELLS OF LILY

作　　者：徐涛[1] 张晓红[1] 聂秀菀[1] 郑国锠[1] 

机构地区：[1]兰州大学细胞生物学研究室,兰州730000

出　　处：《Acta Botanica Sinica》1997年第1期11-15,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金;高校博士点基金

摘　　要：参照动物细胞核骨架的研究方法,用整装电镜技术和DGD包埋-去包埋技术研究了选择性抽提的和完整的百合(Lilium davidii var. willmottiae (Wilson) Roffill)花粉母细胞。结果表明,住减数分裂前期Ⅰ,百合花粉母细胞核内存在一个精细的非染色质纤维——核骨架。该网络由5～15nm的纤维交织而成,广泛地分布于细胞核内。这些核骨架有的分布于染色体间,有的分布于染色体周围,并与染色体和核仁相连。随着减数分裂时间的推移,染色体(质)间核骨架纤维逐渐减少,染色体(质)周围的核骨架纤维逐渐增多,并与染色体内部的纤维结构相连,表明核骨架一方面为染色体拓扑变化提供一个空间支架,另一方面也可能参与了染色体骨架的构建。The nuclear matrix (NM) of pollen mother cells (PMC) of Lilium davidii var. willmot-tiae (Wilson) Roffill was studied using selective extraction, embeddment-free electron microscopy and whole mount cell preparation. A complex internal substructure, which is similar to the nuclear matrix in vertebrate cells, was observed in the nucleus of PMCs at prophase I . These nuclear matrices , comprising delicate protein filaments, spreaded over the nuclear region. The diameters of NM filaments were in the range of 5 - 15 ran. Nucleoli and chromosomes were found to be connected with the NM filament network. The NM was proposed to offer a framework for the topological change of chromatin/chromosome, and might be involved in the construction of the scaffold of metophase chromosome.

关 键 词：百合 花粉母细胞 核骨架 超微结构 

分 类 号：Q949.718.2[生物学—植物学]