PrP八肽重复突变体对细胞生长影响的探讨  

Preliminary analyses for influence of mutant PrPs with different number of octapeptide

作　　者：韩露[1] 万言珍[1] 韩俊[1] 陈岚[1] 孙力[1] 王小凡[1] 黄银霞[1] 董辰方[1] 姜慧英[1] 董小平[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052

出　　处：《中华实验和临床病毒学杂志》2007年第3期208-210,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金委资助项目（30500018、30571672）;欧盟资助项目（QLRT200001441）;国家科技攻关计划资助项目（2003BA712A04-02）

摘　　要：目的探讨PrP八肽重复突变体对细胞生长的影响。方法将具有遗传性朊病毒病相关不同八肽重复区插入突变的PRM，基因表达载体瞬时转染HeLa细胞，经噻唑蓝比色法（MTT）、Annexin—V／PI双染流式细胞检测。结果多八肽突变PrP蛋白对细胞的毒性作用较野生型明显增强。多八肽突变PrP较野生型更容易诱导凋亡发生，多八肽突变组早、晚期凋亡率均高于野生组。结论遗传性朊病毒病相关多八肽突变PrP蛋白可能较野生型更易诱导细胞凋亡发生。Objective The present study was conducted to understand the effects of PrP in different octapeptide repeats on proliferation of HeLa cells. Methods and Results Mutant PrPs with octapeptide repeat insertion were transiently expressed in HeLa cells and their results of MTT assay showed stronger cytotoxic effect On the proliferation of cells than wild-type PrP. Annexin V/PI assay also demonstrated that the expression of mutant PrPs was much easier to induce apoptosis than wild-type in HeLa cells. The percentage of both early and late stage apoptosis in mutant groups were significantly higher than that of wild type. Conclusion These data suggest that the expression of mutant PrPs associated with familial CJD is much easier to induce apoptosis in cultured cells than expression of wild type PrP.

关 键 词：朊病毒 肽类 噻唑蓝比色法 细胞凋亡 

分 类 号：R3[医药卫生—基础医学]