Ad5F35-LMP2重组腺病毒免疫效果的研究  

Immune responses induced by recombinant adenovirus AdSF35-LMP2 in rhesus monkeys

作　　者：莫武宁[1] 周玲[2] 吴小兵[2] 王湛[2] 唐安洲[1] 黄光武[1] 余双庆[2] 王琦[2] 叶树清[2] 杜海军[2] 曾毅[2] 

机构地区：[1]南宁广西医科大学第一附属医院,530021 [2]中国疾病预防控制中心病毒病所国家重点实验室

出　　处：《中华实验和临床病毒学杂志》2007年第3期226-228,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家“863”重大资助项目（2006AA02A229）;国家“973”资助项目（2004C8518806）

摘　　要：目的了解含有EB病毒潜伏膜蛋白2的非复制型重组腺病毒（Ad5F35-LMP2），免疫恒河猴的特异性细胞和体液免疫的效果。方法分别使用高剂量（1．5×10^10TCID50/只）、中剂量（1．5×10^9TCID50/只）、低剂量（1．5×10^8TCID50/只）Ad5F35-LMP2重组腺病毒，同时设对照组（PBS4．0 ml／只）。肌内注射免疫恒河猴，每个月一次，共免疫3次，第0、4、8、12周时使用Elispot方法检测猴外周血EBV—LMP2细胞毒性T细胞应答，同时应用免疫酶方法检测血清中LMP2抗体。结果3个剂量Ad5F35-LMP2腺病毒免疫恒河猴均可以诱导出有效的细胞免疫应答及一定的抗体应答，免疫应答水平的高低与病毒剂量的高低有一定的关系，较高剂量产生的细胞及体液免疫应答水平比低剂量的高。结论Ad5F35-LMP2非复制型重组腺病毒疫苗可以有效的诱导恒河猴产生EBV—LMP2特异性细胞和体液免疫反应。Objective To observe the specific cellular and humoral immune responses after immunization with recombinant adenovirus AdSF35-LMP2 in rhesus monkeys. Methods Sixteen rhesuses were immunized with AdSF35-LMP2 through intra-muscular injection in three groups, high dosage group （1.5 × 10^10 TCID50/rhesus）, medium dosage group （1.5 × 10^9 TCID50/rhesus）, low dosage group （1.5 × 10^8 TCID50/rhesus） and the last group was control （PBS 4 ml/rhesus） .They were totally immunized three times at intervals of one month .The EBV-LMP2 specific cellular immune responses were tested during the 0, 4, 8, 12 weeks by Elispot after immunization respectively.And the titers of anti-LMP2 antibody were tested by EIA at the same time, Results EBV-LMP2 specific cellular and humoral immune responses which were induced by recombinant adenovirus AdSF35-LMP2 can be found in all the three dosage groups. The potency of immune responses was related with the dosage of immunization. Higher dosage elicited more potent immune response. Conclusion The recombinant adenovirus Ad5F35-LMP2 could elicit LMP2 specific cellular and humoral immune responses in rhesus.

关 键 词：重组 遗传 腺病毒 人 疱疹病毒4型 人 膜蛋白质类 恒河猴 免疫 细胞 

分 类 号：R3[医药卫生—基础医学]