水杨酸钠慢性给药对小鼠下丘核神经元超微结构及听反应特性的影响  

Effects of sodium salicylate on ultrastructures and hearing response properties of inferior colliculus neurons in mice

作　　者：尹时华[1] 唐安洲[1] 仁毅[1] 谭颂华[1] 陈平[1] 蔡红武[1] 谢利红[1] 

机构地区：[1]广西医科大学第一附属医院耳鼻咽喉科,南宁530021

出　　处：《中华耳科学杂志》2007年第3期253-258,共6页Chinese Journal of Otology

基　　金：广西医科大学"博士启动基金"项目(304156);广西自然科学基金(桂科自0728134);广西壮族自治区卫生厅资助项目(项目编号Z2007043)

摘　　要：目的探讨水杨酸钠给药对小鼠下丘核神经元超微结构及听反应特性的影响。方法将36只成年健康昆明小鼠分对照组、水杨酸钠给药组(450mg.kg-1.d-1)和水杨酸钠(450mg.kg-1.d-1)+利多卡因(10mg.kg-1.d-1)组三组进行实验。经腹腔注射给药,15d后进行检测。采用透射电镜观测下丘核超微病理学改变;采用细胞外电生理记录技术研究下丘核区神经元在水杨酸钠给药后强度-发放率函数、强度-潜伏期函数和频率调谐曲线的变化。结果(1)水杨酸钠给药使下丘核神经元超微结构,如胞核、核周间隙、核糖体粗面内质网、和胞浆内部分线粒体等发生了明显的病理变性;水杨酸钠+利多卡因给药后下丘核神经元细胞核、核仁、线粒体等病变较水杨酸钠给药组明显减轻。(2)与对照组比较水杨酸钠给药使发放率函数不规则类型、潜伏期函数非单调型和宽阔型神经元调谐曲线类型明显增加(P<0.01,P<0.01,P<0.01);水杨酸钠+利多卡因给药可明显逆转水杨酸钠引起的上述听反应特性的改变。结论水杨酸钠引起下丘核神经元电生理特性发生改变的物质基础可能就是源于水杨酸钠对下丘核神经元超微结构的影响,利多卡因对这种损伤有一定的保护作用。　Objective To study the effects of sodium salicylate on ultrastructure and hearing response properties of inferior colliculus neurons in mice. Methods Thirty-six Kunming mice were divided into three groups（A-control group, B-salicylate group, C-salicilate plus lidocaine group）. The ultrastructures were examined by electronic microscopy. The intensity-rates functions, intensity-latency functions and frequency-tuning curves were recorded by extracellular electrophysiological recording techniques. Results （1） The ultrastructure of inferior colliculus neurons in mice, such as the cell nucleus, perinuclear space, rough endoplasmic reticulum, cytoplasm surrounding the cell nucleus developed marked degeneration after sodium salicylate was given and the changes were obviously meliorated after combined administration of sodium salicylate plus lidocaine. （2） The anomalous intensity-discharing rate functions, the monotonic intensity-latency functions increased markedly, while the frequency-tuning curves were broader than that of control group（P〈0.01,P〈0.01,P〈0.01） after sodium salicylate administration. The above featured hearing response properties were reversed when lidocaine was given. Conclusion The results suggested that the ultrastructural changes after salicylate administration underlie the electrophysiological changes. Lidocaine could reduce these damages induced by salicylate.

关 键 词：水杨酸钠 小鼠 下丘核 听反应 超微结构 

分 类 号：Q42[生物学—神经生物学] R338.3[生物学—生理学]