牙本质磷蛋白mRNA原位杂交方法的建立  

Establishing of in situ hybridization method for the expression of dentin phosphoprotein mRNA

作　　者：张敬雷[1] 

机构地区：[1]解放军175医院.厦门大学附属东南医院口腔科,福建漳州363000

出　　处：《临床口腔医学杂志》2007年第10期598-600,共3页Journal of Clinical Stomatology

摘　　要：目的:建立检测牙本质磷蛋白(DPP)mRNA表达的原位杂交方法。方法:选用发育各阶段的牙胚、牙齿和体外培养的MDPC-23成牙本质细胞为对象,采用地高辛标记的寡核苷酸探针的原位杂交方法。结果:DPP mRNA在牙胚与牙齿中的成牙本质细胞、前成釉细胞和体外培养的成牙本质细胞存在阳性表达。结论:设计的探针敏感性高,特异性高,所建立的原位杂交方法是研究牙本质发育和损伤修复的良好方法。Objective：To establish method of in situ hybridization for the expression of dentin phosphoprotein. Method： Developing tooth germ or teeth and in vitro cultured mouse MDPC-23 odontoblasts were selected. Oligonucleotide probe labeled with digoxigenin were used in in situ hybridization. Result： DPP mRNA could be detected in the odontoblast and preameloblasts in developing tooth germ or teeth and in vitro cultured mouse MDPC-23 odontoblasts. Conclusion： The DPP oligonucleotide probe we designed showed sensitiyity and specificity. In situ hybridization method we established were a good method to study on tooth development and pulp repair after injury.

关 键 词：牙本质磷蛋白 原位杂交 牙胚 寡核苷酸探针 MDPC-23细胞 

分 类 号：Q813.1[生物学—生物工程]