下呼吸道分离白色假丝酵母菌基因型、药敏试验和临床相关因素分析  

Genotyping,Antifungal Susceptibility and Clinic Analysis of 60 Strains of Candida albicans Isolated from Lower Respiratory Tract

作　　者：黄静[1] 张天托[1] 朱家馨[1] 周宇麒[1] 刘慧[1] 谈淑卿[1] 

机构地区：[1]中山大学附属第三医院,广东广州510630

出　　处：《中华医院感染学杂志》2007年第10期1210-1213,1221,共5页Chinese Journal of Nosocomiology

摘　　要：目的了解医院近两年来下呼吸道分离白色假丝酵母菌的基因分型和不同基因型药敏试验结果与临床相关因素的关系。方法以随机扩增PCR对60例下呼吸道分离白色假丝酵母菌进行基因分型和药敏试验,分析感染患者的临床资料。结果下呼吸道分离的60株白色假丝酵母菌基因型可分为5型:A型30株(50.0%),B型13株(21.7%),C型10株(16.7%),D型5株(8.0%),E型2株(3.0%);方差分析表明,前3种基因型白色假丝酵母菌对氟康唑、5-氟胞嘧啶敏感性差异有统计学意义(P<0.05);A型白色假丝酵母菌与B型和C型对氟康唑的敏感性差异有统计学意义(P<0.05);B型对5-氟胞嘧啶的敏感性与A型和C型差异有统计学意义(P<0.05);感染C型白色假丝酵母菌的患者死亡率与A、B型比较差异有统计学意义。结论随机扩增PCR法简捷、特异性强、重复性好,可作为白色假丝酵母菌的分型研究;白色假丝酵母菌对不同的药物敏感性可能与特定基因型有一定相关性,其毒力也可能有所不同。OBJECTIVE To study the relationships among the genotypes of the Candida albicans, the antifungal susceptibility and the clinical characteristics of the patients. METHODS Sixty clinical strains collected from lower respiratory tract infections in adults were genotyped by arbitrary primed polymerase chain reaction fingerprinting. E-test method was applied to test the C. albicans. Clinic analysis was done. RESULTS Genotyping is showed that 60 clinical isolates could be classified into 5 genotypes., genotype A （30 strains, 50%）, genotype B （13 strains, 21.7%）, genotype C （10 strains, 16.7%）, genotype D （5 strains, 8%）, and genotype E （2 strains,3%）. The multi-factorial analysis was shown as follows： there were differences among susceptibilities to FCZ and 5-flucytosine of the three genotypes （A, B, C） of C. albicans（P〈0. 05）. The susceptibility to FCZ of genotype A was lower than B and C （P〈0.05）. But genotype B was more resistant to 5-FC（P〈0.05） and the mortality of genotype C patients was higher than others. CONCLUSIONS Arbitrary primed polymerase chain reaction is a practical, reliable and economical genotype method. It suggested that a higher degree of genetic correlation exist between resistant patterns and its PCR profiles of C. albicans. And the virulence of different C. albicans may be different.

关 键 词：下呼吸道感染 白色假丝酵母菌 基因分型 药敏试验 临床分析 

分 类 号：R379.4[医药卫生—病原生物学]