草酸钙尿石症患者肾组织VKDC基因表达及其意义  

Gene expression of vitamin k dependent carboxylase in patients with calcium oxalate urolithiasis

作　　者：杨俊[1] 刘继红[1] 郭小林[1] 王涛[1] 王军凯[1] 樊龙昌[1] 王少刚[1] 叶章群[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030

出　　处：《临床泌尿外科杂志》2007年第10期736-738,共3页Journal of Clinical Urology

基　　金：国家自然科学基金资助(编号30400442)

摘　　要：目的:探讨草酸钙尿石症患者肾组织中维生素K依赖性羧化酶(VKDC)基因mRNA表达水平及其与草酸钙尿石形成的关系。方法:以TaqMan实时荧光定量PCR法检测19例草酸钙尿石症肾组织(尿石组)与21例非尿石肾组织(对照组)标本中VKDC基因mRNA的表达,采用2-△△Ct法比较两组标本中VKDC基因mR-NA表达的差异。结果:尿石组与对照组荧光定量检测△Ct值分别为8.996±1.667与6.023±1.234(P<0.05),尿石组VKDCmRNA表达明显降低。结论:草酸钙尿石症患者肾组织中VKDC基因表达明显下降,其表达水平的变化可能与草酸钙结石形成有关。Objective：To study the gene expression of vitamin k dependent carhoxylase （VKDC） in patients with calcium oxalate urolithiasis and evaluate the correlation between the quantity of VKDC and the formation of calcium oxalate calculus. Methods： Using Taqman real-time reverse transcription-polymerase chain reaction （RT-PCR） and 2^△△Ct, method, the expression of VKDC mRNA were evaluated in renal tissue specimens from patients with calcium oxalate urolithiasis （urolithiasis group, n= 19） and non-urolithiasis （control group, n= 21）. Glyceraldehyde 3-phosphate dehydrogenase （GAPDH） was used as the housekeeping gene. Results： The amount of VKDC mRNA in urolithiasis group was lower than in the controls, indicated by significantly higher delta threshold cycles （△Ct） in RT-PCR for the VKDC gene in the urolithiasis renal tissue, with mean ± SD of 8. 996±1. 667 and 6. 023±1. 234（P〈0. 01）, respectively. VKDC mRNA was on average 7.86-fold（range, 2. 47 to 24.93-fold）underexpressed in urolithiasis group compared to controls. Conclusions：These data demonstrate that the gene expres- sion of VKDC is decreased in renal tissues of patient with calcium oxalate urolithiasis, and may he involved in the formation of calcium oxalate calculus.

关 键 词：维生素K依赖性羧化酶 草酸钙结石 尿石症 

分 类 号：R691.4[医药卫生—泌尿科学]