Fe^(2+)-邻苯二胺-H_2O_2催化体系分光光度法测定痕量过氧化氢  

Spectrophotometric Determination of Trace Hydrogen Peroxide Using Fe^(2+)-O-Phenylenediamine-Oxydol

作　　者：梁爱惠[1] 王娜[2] 

机构地区：[1]桂林工学院材料与化学工程系,广西桂林541004 [2]广西师范大学环境与资源学院,广西桂林541004

出　　处：《桂林工学院学报》2007年第3期402-405,共4页Journal of Guilin University of Technology

基　　金：广西自然科学基金资助项目(桂科基0575042)

摘　　要：在pH2.64的HCl-NaAc缓冲溶液中,Fe2+催化H2O2产生.OH,.OH的量与加入H2O2的量成正比,.OH能氧化邻苯二胺(OPDA)生成2,3-二氨基吩嗪,此产物在450 nm处有一最大吸收峰,其吸光度值随着加入过氧化氢量的增加而增加,与过氧化氢的浓度在0.018~1.03μg/mL范围呈良好的线性关系,检出限为0.001 4μg/mL H2O2.据此建立了一个灵敏、简便、快速、准确、选择性好,测定痕量过氧化氢含量的分光光度法,该法已用于水样分析,结果满意.In pH 2.64 HCl - NaAc buffer solutions, Fe^2 ＋ reacts with H2O2 to produce hydroxyl radical. The amounts of hydroxyl radical are propotional to hydrogen peroxide. Then hydroxyl radicals from fenton reaction oxidize o-phenylenediamine to form 2, 3-diaminophenazine. This production has a peak absorption at 450 nm. When hydrogen peroxide is added, the peak increases sharply. The results show that the increased absorbance value at 450 nm is linear to the hydrogen peroxide concentration in the range of 0. 018 - 1.03 μg/mL. The detection limit is 0. 001 4 μg/mL. A new spectrophotometric method is proposed for the determination of H2O2 in synthetic samples and water samples, with the advantages of high sensitivity, simplicity, rapidity, accuracy and selectivity.

关 键 词：过氧化氢 邻苯二胺 FENTON反应 分光光度法 

分 类 号：O657.32[理学—分析化学]