荧光标记3个miniSTR基因座复合扩增系统的建立  

The establishment of fluorescent multiplex set for three miniSTR loci

作　　者：王会品[1] 刘超[2] 陈晓晖[2] 刘宏[2] 刘长晖[2] 

机构地区：[1]中山大学中山医学院法医学系 [2]广州市公安局刑科所,广东广州510030

出　　处：《中国法医学杂志》2007年第5期308-310,共3页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30400519);广州市重大科技攻关项目(2005Z1-E0051)

摘　　要：目的建立扩增片段<135bp,包括D5S818,D8S1179,D16S539 3个miniSTR基因座复合扩增系统。方法采用不同荧光染料标记引物,通过PCR扩增,利用ABI 3100遗传分析仪进行片段长度分析,对100份无关个体血样,10个家系样本以及30份高度降解检材进行检测。结果本系统DNA分型结果与AmpFLSTR Identifiler试剂盒完全一致,且灵敏度高于AmpFLSTR Identifiler试剂盒。结论本系统可以应用于个人识别和亲权鉴定,为降解DNA样本分型提供了新的方法。Objective To establish a miniSTR multiplex set which includes three STR loci D5S818, D8S1179 and D16S539, whose amplified fragments shorter than 135bp. Methods After amplification with fluorescence labeled primers, the amplified products from 100 unrelated individuals, 10 genealogies, as well as 30 highly degraded specimens, were analyzed by ABI 3100 Genetic Analyzer. The genotypes obtained by the miniSTR multiplex set were consistent with that by the AmpFLSTR Identifiler kit, and the former had a higher sensitivity than the latter. Conclusion The miniSTR multiplex set is useful in personal identification and paternity test, especially in the analysis of degraded DNA sample.

关 键 词：法医物证学 MINISTR 复合扩增 D5S818 D8S1179 D16S539 

分 类 号：D919[医药卫生—法医学]