游离脂肪酸调节脂肪细胞脂联素受体基因的表达  

Free fatty acids regulate expression of adiponectin receptor gene in adipocytes

作　　者：温宇[1] 王宏伟[1] 吴静[1] 卢慧玲[1] 胡秀芬[1] 夏治[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,武汉430030

出　　处：《中华内分泌代谢杂志》2007年第5期454-456,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金(30470645)

摘　　要：采用RT-PCR法检测以游离脂肪酸(FFA)孵育的(前)脂肪细胞中脂联素受体(AdipoR) mRNA的表达规律。结果表明3T3-L1脂肪细胞AdipoR1和AdipoR2基因表达呈分化依赖性增强。油酸仅对前脂肪细胞AdipoR的表达起抑制作用,而高浓度棕榈酸能抑制(前)脂肪细胞AdipoR的表达。[ Summary] 3T3-L1 preadipocytes were induced to differentiate and 3T3-L1 adipocyte or preadipocytes were incubated with oleate or palmitate overnight. RT-PCR was used to measure adiponectin receptor （AdipoR） 1 and AdipoR2 mRNA levels. The results showed that the AdipoR1 and AdipoR2 expressions were differentiationdependent. Oleate only suppressed AdipoR mRNA expression in preadipocyte but not in adipocyte. However, high concentration of palmitate reduced AdipoR mRNA expression in both 3T3-L1 preadipocyte and adipocyte.

关 键 词：3T3-L1细胞 脂肪酸非酯化 受体 脂联素 

分 类 号：R587.1[医药卫生—内分泌] R589.2[医药卫生—内科学]