人精浆酸性磷酸酶对小鼠T淋巴细胞产生IL-2的影响  

The Effect of Acid Phosphatase from Human Semen on IL-2 Production by Mouse T Lymphocyte

作　　者：程丽萍[1] 李伟毅[1] 陈广洁[1] 朱云凤[1] 

机构地区：[1]上海第二医科大学上海免疫研究所,上海200025

出　　处：《生殖与避孕》1997年第1期19-22,共4页Reproduction and Contraception

基　　金：上海市自然科学基金

摘　　要：本文研究了从正常人精浆中分离纯化的酸性磷酸酶（ACP）对BALB／c小鼠脾脏细胞来源的T淋巴细胞产生IL-2的影响。本文采用MTT生物学测定法测IL-2活性。实验证明：人糖浆ACP对小鼠T淋巴细胞产生IL-2有明显的抑制作用（P＜0．05），并且抑制作用随ACP剂量的增加而增强。这说明了精浆中大量ACP是构成精浆免疫抑制功能的重要组成成份之一。The purpose of this study is to observe the effect of acid phosphatase (ACP) purified from human sernen on the IL-2 production by BALB/c mouse spleen T lymphocyte. MTT biological assay was used to measure the activity of IL-2. It has been proved that the production of IL - 2by mouse spleen T lymphocyte was significantly suppressed by human semen ACP (P<0.05)witb dose-dependent pattern. It has been suggested that a great amount of ACP existing in hu-man semen migbt be the main composition of immunosuppressive substance of human semen.

关 键 词：人精浆 酸性磷酸酶 淋巴细胞 白细胞介素2 

分 类 号：R392.12[医药卫生—免疫学] R339.21[医药卫生—基础医学]