基于层层静电吸附天青Ⅰ和纳米金固定过氧化物酶生物传感器的研究  

Hydrogen peroxide biosensor based on Azure Ⅰ and nano-Au through layer by layer electrostatic adsorption

作　　者：李文娟[1] 袁若[1] 柴雅琴[1] 王娜[1] 陈时洪[1] 高凤仙[1] 杨淦[1] 

机构地区：[1]西南大学化学化工学院重庆市现代分析化学重点实验室,重庆400715

出　　处：《分析试验室》2007年第10期9-13,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(29705001);重庆市自然科学基金(CSTC-2004BB4149;2005BB4100);西南大学博士基金(SWNUB2004021);高新技术培育基金(XSGX02)项目资助

摘　　要：在玻碳电极(GCE)上,构造了一种以对氨基苯磺酸电聚合膜(PABSA)为基底,利用层层静电自组装技术固定多层天青Ⅰ(AI)和纳米金(nano-Au)制备的复合薄膜(nano-Au/AI)n,然后通过静电吸附辣根过氧化物酶(HRP)制得过氧化氢生物传感器[HRP/(nano-Au/AI)n/PABSA/GCE]。采用循环伏安法和计时电流法考察了该传感器的电化学性质,并且研究了该修饰电极对H2O2的催化还原作用。在优化的实验条件下,该传感器的响应电流与其浓度在3.5×10-6～3.6×10-3mol/L范围内呈现良好的线性关系,检出限为1.2×10-6mol/L。该传感器的米氏常数为1.5 mmol/L,表明所固定的酶具有较高的生物活性。A sensitive amperometric biosensor for the determination of H2O2 has been constructed. Layer-by-layer selfassembly technique is used to form Azure Ⅰ and nano-Au muhilayer films （nano-Au/Al）n onto a poly （p-aminobenzene sulfonic acid）-modified glassy carbon electrode, then the positively charged horseradish peroxidase is successfully immobilized on the gold nanoparticles surface. The electrocatalytic behavior of the modified electrode to H2O2 was investigated by cyclic vohammetry and chronoamperometry. The experiments showed that the modified electrode had excellent electrocatalytic activity for the reduction of H2O2. The linear range of this biosensor is 3.5 × 10^-6 - 3.6 × 10^-3 mol/L with a detection limit of 1.2 × 10^-6 mol/L （S/N = 3）. In this experiment, HRP can retain a higher bioactivity with the Michaelis-menten Constant of 1.5 mmol/L.

关 键 词：对氨基苯磺酸 天青Ⅰ 纳米金 辣根过氧化物酶 生物传感器 层层静电自组装技术 

分 类 号：O657.1[理学—分析化学]