反相高效液相色谱法测定玉米中玉米赤霉烯酮  

Determination of zearalenone in corn by high performance liquid chromatography with fluorescence detection

作　　者：康维钧[1] 王玉平[1] 杨福江[2] 谈敦芳[1] 计融[3] 

机构地区：[1]河北医科大学公共卫生学院,石家庄050017 [2]邢台医学高等专科学校预防医学教研室,邢台054000 [3]中国疾病预防控制中心营养与食品安全所,北京100050

出　　处：《分析试验室》2007年第10期66-68,共3页Chinese Journal of Analysis Laboratory

基　　金："十五"国家科技重大专项<食品安全关键技术>之"食品中有害微生物及生物毒素限量研究与标准制定"课题(2001BA804A3205)项目资助

摘　　要：建立了反相高效液相色谱法检测玉米中玉米赤霉烯酮的分析方法。该方法的标准曲线回归方程为y=2.97×103+5.23×104ρ(r=0.9998);玉米样品3个水平的加标回收率分别为84.8%,86.7%和89.2%;相对标准偏差分别为4.0%,5.2%和4.4%;加标浓度为50 ng/g的玉米样品日内相对标准偏差为0.69%;玉米样品日间相对标准偏差为2.6%;方法检出限为3.0 ng/g。A reversed-phase HPLC method for determining zearalenone in com was developed. The linear equation of the method was y = 2.97 × 10^3 ＋ 5.23 × 10^4x （ r = 0.9998）. The recoveries of zearalenone after spiked with 50, 75 and 100 ng/g to com were 84.8%, 86.7% and 89.2%; and the relative standard deviations were 4.0%, 5.2% and 4.4%, respectively. The coefficients of variation intra-day of spiked com were O. 69%, the coefficients of variation inter-day of spiked com was 2.6%. The detection limit was 3.0 ng/g based on a signal-to-noise ratio of 3：1.

关 键 词：玉米 玉米赤霉烯酮 高效液相色谱 

分 类 号：O557.7[理学—热学与物质分子运动论]