内皮素对人肾小球系膜细胞表达细胞粘附分子的影响  

Upregulation of cell adhesion molecules by endothelin-1 on human mesangial cells in vitro

作　　者：林京梅[1] 谌贻璞[1] 高进[1] 王海燕[1] 

机构地区：[1]北京医科大学第一附属医院肾内科,肾脏病研究所,100034

出　　处：《中华肾脏病杂志》1997年第2期67-69,共3页Chinese Journal of Nephrology

基　　金：国家自然科学基金

摘　　要：目的 探讨内皮素-1(ET1-1)对体外培养的人肾小球系膜细胞表达细胞间粘附分子-1(ICAM-1)和血管细胞粘附分子-1(VCAM-1)的调节作用。方法 利用Northern杂交技术和细胞ELISA方法分别从mRNA和蛋白质两个水平观察ICAW-1和VCAM-1的变化。结果 在正常培养条件下,人肾小球系膜细胞仅低水平表达一定量的ICAM-1、VCAM-1的mRNA和蛋白质;ET-1(10^(-7)mol)刺激后,这两种细胞粘附分子的mRNA表达迅速上调,且随后其相应蛋白质表达也显著增加并呈时间依赖性。结论 提示ET-1对粘附分子表达的促进作用可能在传小球疾病的发生中具有重要意义。Objective To study the expression of intercellular adhesion molecule-1 ( ICAM-1 ) and vascular cell adhesion molecule-l(VCAM-L) on human mesangial cells (HMC) after stimulation by endothelin-1 (ET-1). Methods The ex-pression of ICAM-1 and VCAM-1 was detected at mRNA and protein levels by using Northern bolt and cell ELISA respectively. Results There was basic mRNA and protein expression of ICAM-1 and VCAM-1 on HMC in the control groups without ET-1. ET-l(10-7mol)augmented the mRNA expression of ICAM-1 and VCAM-1 with the maximum at 4 -8 hr. Increased protein expression of ICAM-1 and VCAM-1 was followed thereafter in a time-depended manner. Conclusion The upregulated expression of ICAM-1 and VCAM-1 by ET-1 may have an important role in the development of glomerulonephritis.

关 键 词：细胞间粘附分子 血管 内皮素 肾小球系膜细胞 

分 类 号：R692.603[医药卫生—泌尿科学]