高效液相色谱法同时测定灭鼠毒饵中杀鼠灵、溴敌隆、大隆的含量  

Determination of concentration of warfarin,bromadiolone,brodifacoum in the poison bait by high performance liquid chromatography

作　　者：刘思洁[1] 方赤光[1] 张博[1] 李青[1] 张丽薇[1] 

机构地区：[1]吉林省卫生监测检验中心理化检验所,长春130062

出　　处：《中国媒介生物学及控制杂志》2007年第5期379-381,共3页Chinese Journal of Vector Biology and Control

摘　　要：目的建立高效液相色谱法（HPLC）同时测定毒饵中杀鼠灵、溴敌隆、大隆3种抗凝血灭鼠剂的含量。方法在C18色谱柱，以甲醇＋0．1％乙酸（90＋10）为流动相，检测波长为265nm，柱温为室温下进行检测。结果3种灭鼠剂在5．0～300．0μg／ml浓度之间呈现良好的线性关系，杀鼠灵：Y=2．58×10^7x-1．23×10^5，r=0．9996；溴敌隆：Y=2．09×10^7x＋1．56×10^5，r=0．9995；大隆：Y=1．79×10^7x＋1．02×10^5，r=0．9992；相对标准偏差为1．03％～2．16％，回收率在93．7％～98．8％之间。结论HPLC法操作简便、准确、快速，可同时对这3种抗凝血灭鼠剂含量进行检测。Objective To establish a high performance liquid chromatography method for determination of the concentration of warfarin, bromadiolone, brodifacoum in the poison bait. Methods Warfarin, bromadiolone and brodifacoum were separated on a C18 column using methanol （50%）： 0.1% aceticacid （50%） as the mobile phase at a flow rate of 1.0 ml/min, and detection wave 265 nm. Results The linear range were 5.0μg/ml to 300.0 μg/ml, warfarin Y=2.58×10^7x-1.23×10^5,r=0.9996;bromadiolone Y=2.09×10^7x＋1.56×10^5,r=0.9995;brodifacoum Y=1.79×10^7x＋1.02×10^5,r=0.9992; the relative standard deviation were 1.03% to 2.16% and the rate of recovery were 93.7% to 98.8% . Conclusion The method established in the present paper is simple, precise, fast and suitable for determination the concentration of warfarin, bromadiolone, brodifacoum in poison bait.

关 键 词：高效液相色谱法 灭鼠毒饵 杀鼠灵 溴敌隆 大隆 

分 类 号：S482.55[农业科学—农药学]