Immune Responses and Histopathological Changes in Rabbits Immunized with Inactivated SARS Coronavirus  

作　　者：Chuan-hai ZHANG Xin-jian LIU Yi-fei WANG Jia-hai LU Huan-ying ZHENG Sheng XIONG Mei-ying ZHANG Qiu-ying LIU 

机构地区：[1]Biomedicine Research ＆ Development Center, Jinan University, Guangzhou 510630, China [2]Department of Chemistry and Biology, West Anhui University, Lu-an 237012, China [3]The First People＇s Hospital Shanghai Jiaotong University, Shanghai 200080, China

出　　处：《中国病毒学》2007年第5期360-365,共6页Virologica Sinica

基　　金：Joint funds of National Natural Science Foundation of China (U0632010);Program of Guangdong Provincial Key Lab of Bioengineering Medicine (51207026).

摘　　要：To evaluate the immunogenicity of inactivated SARS coronavirus(SARS-CoV),three groups of rabbits were immunized three times at 2-week intervals with inactivated vaccine + adjuvant,adjuvant,and normal saline respectively.Eight batchs of serum were sampled from the auricular vein at day 7 to day 51,and specific IgG antibody titers and neutralizing antibody titers were detected by indirect ELISA and micro-cytopathic effect neutralizing test.Antibody specificity was identified by proteinchip assay.Histopathological changes were detected by H&E staining.The results showed that,rabbits in the experimental group immunized with inactivated SARS-CoV all generated specific IgG antibodies with neutralizing activity,which suggested the inactivated SARS-CoV could preserve its antigenicity well and elicit an effective humoral immune responses.The peak titer value of specific IgG antibody and neutralizing antibody reached 1:40960 and 1:2560 respectively.In the experimental group,no obvious histopathological changes was detected in the H&E stained slides of heart,spleen,kidney and testis samples,but the livers had slight histopathological changes,and the lungs presented remarkable histopathological changes.These findings are of importance for SARS-CoV inactivated vaccine development.To evaluate the immunogenicity of inactivated SARS coronavirus（SARS-CoV）,three groups of rabbits were immunized three times at 2-week intervals with inactivated vaccine ＋ adjuvant,adjuvant,and normal saline respectively.Eight batchs of serum were sampled from the auricular vein at day 7 to day 51,and specific IgG antibody titers and neutralizing antibody titers were detected by indirect ELISA and micro-cytopathic effect neutralizing test.Antibody specificity was identified by proteinchip assay.Histopathological changes were detected by H＆E staining.The results showed that,rabbits in the experimental group immunized with inactivated SARS-CoV all generated specific IgG antibodies with neutralizing activity,which suggested the inactivated SARS-CoV could preserve its antigenicity well and elicit an effective humoral immune responses.The peak titer value of specific IgG antibody and neutralizing antibody reached 1：40960 and 1：2560 respectively.In the experimental group,no obvious histopathological changes was detected in the H＆E stained slides of heart,spleen,kidney and testis samples,but the livers had slight histopathological changes,and the lungs presented remarkable histopathological changes.These findings are of importance for SARS-CoV inactivated vaccine development.

关 键 词：免疫反应 组织病理学 兔 急性呼吸综合症 冠状病毒 SARS 疫苗 

分 类 号：R373[医药卫生—病原生物学] R392[医药卫生—基础医学]