瘢痕疙瘩克隆状态的分析  

Analysis of clone state of keloid scars

作　　者：高扬[1] 高凤山[1] 李虎[1] 董继英[1] 

机构地区：[1]苏州大学附属第四医院整形外科,江苏无锡214000

出　　处：《中国美容整形外科杂志》2007年第5期357-359,共3页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的通过对人雄激素受体(HUMARA)基因甲基化状态的分析,检测瘢痕疙瘩中X染色体的失活方式,判断瘢痕疙瘩的克隆状态。方法选择69例女性患者,瘢痕疙瘩组34例、正常皮肤组35例,分别提取DNA,经甲基化敏感性限制性核酸内切酶HhaⅠ消化后,对HUMARA基因片段进行PCR扩增并电泳,通过观察电泳条带判断两组患者HUMARA基因的克隆状态。结果HUMARA基因的杂合率为85.29%,瘢痕疙瘩组、正常皮肤组的多克隆率分别为72.41%、92.86%,两组比较差异有统计学意义(P<0.05)。结论多克隆样本的出现,表明瘢痕疙瘩不是肿瘤细胞克隆性增殖的结果,而是由接收了异常细胞外信号的内部正常多克隆细胞形成的。此结果有助于未来对确立这一假设异常调整信号在基因和分子水平上的研究,而这一调整信号在普通、异常瘢痕的形成中起着非常重要的作用。Objective To assess the clone state of keloid by analyzing methylated state of human androgen （HUMARA） gene and detecting X- chromosome inactivation pattern. Methods Sixty - nine Thirty - four female patients were invoved in this study, 34 patients with keloids was as the kelois group, 35 patients with normal skin as the normal skin group. Samples were harvested from the both groups, and tissue DNA was extracted, and then digested with methylation sensitive restriction endonuclease Hha Ⅰ . HUMARA fragment was amplified with PCR method and PCR product was electrophoresed. Clonality was assessed by observing the electrophoreslshands. Results Heterozygotic rate of HUMARA was 85.29 %. Polyclonal rates in keloid and normal skin group were 72.41%, 92.86 %, respectively. Differences between them was statistically significant （ P 〈 0.05 ）. Conclusion The presence of polyclonal specimens indicated that keloids was not the result from clonal proliferation of neoplasm cells but the result from intrinsically normal cells that are responding to an abnormal extracellular signal. This result can guide future genetic and rnolecular studies to identify this proposed abnormal regulatory signal, which we expect to be an important regulator of normal and diseased scarring.

关 键 词：瘢痕疙瘩 发病机制 多克隆 成纤维细胞 雄激素受体 

分 类 号：R622[医药卫生—整形外科]