iNOS在扩张皮肤组织中的表达及其意义  

Expression of iNOS in expanded cutaneous tissues

作　　者：蔡震[1] 蒋海越[1] 国冬军[1] 潘博[1] 庄洪兴[1] 

机构地区：[1]中国医学科学院中国协和医科大学整形外科医院外耳再造整形中心,北京100041

出　　处：《中国美容整形外科杂志》2007年第5期386-389,共4页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的研究催化一氧化氮生成的关键酶之一,诱导型一氧化氮合酶(iNOS)在扩张皮肤组织中的表达,并探讨其意义。方法将取自18例行皮肤软组织扩张术患者的扩张皮肤18份标本(扩张组)及自身对照的正常皮肤10份标本(正常组),采用免疫组化方法检测扩张皮肤组织及正常皮肤组织中的iNOS、CD34、增殖性细胞核抗原(PCNA)的表达。结果扩张组中iNOS的表达较正常组的表达显著增强(P<0.05),正常组的在表皮基底层有弱阳性表达,扩张组的在表皮基底层有强阳性表达。扩张组中的真皮乳头微血管数较正常组的显著增多(P<0.05);PCNA标记指数(PLI)无统计学意义(P=0.062>0.05)。扩张组中的iNOS表达水平与血管密度计数(MVD)呈显著正相关(R=-0.715,P<0.05)。与PLI无显著相关性(R=-0.27,P=0.278>0.05)。结论iNOS在扩张皮肤组织中的表达增强,高水平表达的iNOS可能会通过促进扩张皮肤组织中真皮乳头血管的形成而促进皮肤的扩张。Objective To study the expression of inducible nitric oxide synthaze （iNOS） in distended cutaneous tissues and explore its significance. Methods Eighteen expanded skin specimens as expanded group were taken from 18 patients and 10 normal skin specimens as control group were gotten from the same patients. The expressions of iNOS, CD34 and poliferating cell nuclear antigen （PCNA） in both groups were examined with immunohistochemistry staining （IHC）. Results The expression of iNOS was significantly higher in epidermis of expanded cutaneous tissues than that in the control group （P〈0.05）. Expression of iNOS in basal cell layer was weak in control group, however, was strong in the expanded group. The microvessel density （MVD） in dermal paplilla in the expended group was significantly higher than that in the control group （P 〈 0.05） ; and there was no significant difference in PCNA label index （PLI） （ P = 0. 062 〉 0.05 ）. There was significantly positive correlation beween the expression level of iNOS and MVD in expanded group （R = - 0. 715, P 〈 0.05） while no correlation between iNOS and PLI （R = - 0.27, P = 0. 278 〉 0.05）. Conclusion The expression of iNOS was markedly increased in epidermis of patient with expanded cutaneous tissues. The higher expression of iNOS may increase vasoformation of dermal papilla to promote the skin expansion.

关 键 词：皮肤软组织扩张术 诱导型一氧化氮合酶 增殖性细胞核抗原 CD34 

分 类 号：R622[医药卫生—整形外科]