用实时荧光PCR方法定量检测Bt176转基因玉米  

Quantitative Detection of the Genetically Modified Bt176 Maize Using Real-time Fluorescent PCR Method

作　　者：李葱葱[1] 王青山[2] 李飞武[1] 刘娜[1] 康岭生[1] 王晶[1] 赵宁[1] 张明[1] 

机构地区：[1]吉林省农业科学院生物技术研究中心,长春130124 [2]中国热带农业科学院

出　　处：《吉林农业科学》2007年第5期24-27,共4页Journal of　Jilin Agricultural Sciences

摘　　要：以实时荧光PCR技术鉴定商业化种植的转基因玉米Bt176品系。根据转基因玉米Bt176中内源基因Zein和外源基因cry1Ab设计引物及TaqMan荧光探针,成功建立了检测转基因玉米Bt176品系的实时荧光PCR方法。结果表明,应用实时荧光PCR方法检测转基因作物,不仅可以达到品系鉴定的作用,而且可以用于转基因成分的检测。该方法简便迅速,降低了污染机会,为转基因作物及产品的品系检测提供了新方法。The PCR primer and TaqMan fluorescence probe was designed according to me sequences oI endogenous Zein gene and exogenous cryIAb gene, and a real-time fluorescent PCR method was performed for detection of the genetically modified Bt176 maize trains. The results showed that the real-time fluorescent PCR method was more convenient and quickly,less contamination than common PCR. The new method for detection of genetically modified organism was established.

关 键 词：实时荧光PCR Bt176转基因玉米 TaqMan荧光探针 

分 类 号：S513[农业科学—作物学]