环磷酰胺作用下成年大鼠卵巢干细胞因子和mRNA的表达  

Cyclophosphamide-induced ovarian damage and stem cell factor expression in rat ovaries

作　　者：罗璐[1] 杨冬梓[1] 王箴 张清学[1] 莫亚勤[1] 狄娜[1] 

机构地区：[1]中山大学附属第二医院妇产科,广东广州510120 [2]深圳市南山区蛇口人民医院,广东深圳518067

出　　处：《南方医科大学学报》2007年第10期1476-1479,共4页Journal of Southern Medical University

基　　金：卫生部部属(管)医疗机构临床学科重点项目;国家教育部博士点(20050558093);中国博士后基金(20060390755)~~

摘　　要：目的探讨化疗药物对卵巢的损伤及其可能机制。方法将成年SD大鼠分为两组:生理盐水组和环磷酰胺组。大鼠腹腔注射环磷酰胺;8周后处死,固定一侧卵巢,连续切片,观察卵巢形态学改变并计数卵泡数目。免疫组化方法检测干细胞因子(SCF)蛋白在卵巢的表达,RT-PCR半定量法检测对侧卵巢SCF mRNA的表达。结果环磷酰胺可引起卵巢间质的严重损害和局部纤维化的发生。SCF可表达于大鼠卵巢原始卵泡和初级卵泡的卵母细胞,次级卵泡和早期窦卵泡的颗粒细胞也可见SCF的表达。环磷酰胺组注射后8周卵泡颗粒细胞SCF蛋白显著升高,卵母细胞SCF蛋白显著减少(P<0.05);卵巢SCF mRNA显著升高(P<0.05)。结论SCF在化疗卵巢表达的变化有助于进一步阐释化疗药物损伤卵巢的机制。Objective To investigate the possible pathways for ovarian injury after administration of cyclophosphamide in rats. Methods Adult SD rats received a single injection of saline vehicle or chemotherapeutic agent cyclophosphamide, and 8 weeks later, the ovaries were removed, fixed and serially sectioned for pathological examination and ovarian follicle counting. The expression of stem cell factor （SCF） protein was evaluated by immunohistochemistry and immunoreactive score, and SCF mRNA expression determined by RT-PCR in rat ovaries. Results Cyclophosphamide had a detrimental effect on ovarian stromal function and lead to primordial follicle loss. Immunoreactive SCF antigens were expressed on the oocytes in the primordial and primary follicles of rat ovaries, and also in the granulosa cells of the secondary follicles and early antral follicles. There was a higher granulosa SCF, lower oocyte SCF and higher SCF mRNA level in the ovaries of the rats exposed to cyclophosphamide as compared with those in control rat ovaries （P〈0.05）. Conclusion Altered SCF expression in the ovaries of rats exposed to cyclophosphamide can be helpful for understanding the mechanisms for chemotherapeutic drug- induced ovarian damage.

关 键 词：化疗 卵巢 干细胞因子 免疫组化 RT-PCR 

分 类 号：R711.75[医药卫生—妇产科学]