核因子-κB活化对大鼠烧伤早期肺组织表达促炎细胞因子的作用  

Effect of nuclear factor-κB activation on expression of proinflammatory cytokines in rat lung tissues in early stage of burn injury

作　　者：李志清[1] 黄跃生[2] 杨宗城[2] 王甲汉[1] 

机构地区：[1]南方医科大学南方医院烧伤科,广东广州510515 [2]第三军医大学西南医院全军烧伤研究所,重庆400038

出　　处：《南方医科大学学报》2007年第10期1496-1497,1500,共3页Journal of Southern Medical University

基　　金：国家重点基础研究发展规划基金(G1999054202)~~

摘　　要：目的探讨核因子-κB(NF-κB)在大鼠烧伤早期肺组织表达促炎细胞因子中的作用。方法采用Wistar大鼠Ⅲ度35%TBSA烧伤模型。实验分正常对照组、烧伤组、烧伤后吡咯烷二硫代氨基甲酸盐干预组(PDTC组)。大鼠烧伤后1、3、6、12、24h凝胶电泳迁移率分析法检测肺组织NF-κB活性;逆转录-聚合酶链式反应检测TNFα、IL-8 mRNA的表达。结果大鼠烧伤后肺组织NF-κB活性在伤后1h内即迅速增高,并持续增高到伤后24h。伤后肺组织TNFα、IL-8 mRNA表达逐渐增多,6h达高峰。PDTC组NF-κB活性降低,肺组织TNFα和IL-8 mRNA表达均较对照组明显减少。结论严重烧伤可活化肺组织NF-κB,从而介导对细胞因子的合成和释放。提示NF-κB活化在烧伤后脏器组织细胞表达释放细胞因子过程中起重要作用。Objective To investigate the effect of nuclear factor-κB（NF-κB）activation on the expression of proinflammatory cytokines in the lung tissues of rats with early-stage burn injury.Methods Wistar rats were randomly divided into 3 groups,namely the normal control,burn,burn and PDTC treatment groups,and in the latter two groups,the rats were subjected to 35% TBSA full-thickness burns.Activation of pulmonary NF-κB at 1,3,6,12,and 24 postburn hour（PBH）was tested by electrophoretic mobility shift assay,and the expressions of pulmonary tumor necrosis factor α（TNFα）and interleukin-8（IL-8）mRNAs at 3,6,12,and 24 h were detected by RT-PCR.Results Compared to that of the control group,activity of pulmonary NF-κB in burned rats was markedly increased within 1 PBH and kept incrasing till 24 h.Expressions of pulmonary TNFα and IL-8 mRNAs increased gradually,reaching the peak level at 6 PBH,and PDTC could effectively inhibit pulmonary NF-κB activation and expression of the pulmonary cytokines induced by the burn injury.Conclusion Severe burn injury may activate pulmonary NF-κB,which ultimately leads to secretion of cytokines in the lung tissues.

关 键 词：烧伤 肺 核因子-ΚB 

分 类 号：R644[医药卫生—外科学]