胃癌乙酰肝素酶表达和DNA含量流式细胞定量研究  

Investigation on the Expression of Heparanase and DNA Content in Gastric Carcinoma by Flow Cytometry

作　　者：刘颖[1] 杨洪钢[1] 左连富[2] 

机构地区：[1]武警广东总队医院,510507 [2]河北医科大学第四附属医院肿瘤研究所流式细胞室

出　　处：《医学研究杂志》2007年第10期40-43,共4页Journal of Medical Research

摘　　要：目的探讨胃癌中乙酰肝素酶(heparanase)的表达及DNA含量与胃癌临床病理特征的关系。方法应用流式细胞术检测50例胃癌组织、25例癌旁组织和10例正常组织中乙酰肝素酶的表达和DNA含量。结果heparanase在胃癌组、癌旁组、正常组中表达量(FI值)分别为1.86±0.44,1.22±0.45,1.00±0.21,3组间差别有显著性(P<0.05),heparanase在胃癌中的阳性表达率为96%,明显高于癌旁和正常胃黏膜(P<0.01),且与胃癌的浸润深度(P<0.05)、TNM分期(P<0.01)和淋巴结转移相关(P<0.01)。3组的DI值分别为1.26±0.14,1.05±0.14,1.00±0.11,3组间差别有显著性(P<0.05),且与肿瘤的分化程度和淋巴结转移相关(P<0.01)。Heparanase表达的FI值与DI值呈正相关关系(r=0.536,P<0.01)。结论Heparanase在肿瘤的浸润和转移中扮演重要的角色,DNA含量与肿瘤的恶性程度密切相关。heparanase和DNA含量可能是评价胃癌恶性程度和预后预测的重要指标。Objective To investigate the relation of heparanase expression and DNA content to the clinicopathological characteristic in gastric carcinoma. Methods FCM was used to detect the expression of heparanase and DNA content in 50 gastric carcinoma,25 adja- cent carcinoma and 10 normal mucosa. Results FI value of Heparanase in carcinoma group, adjacent carcinoma group and normal muco- sa group was 1.86 ± 0.44,1.23 ± 0.45,1.00 ± 0.21, There was a significant difference between them （ P 〈 0.05 ）. The positive expression rate of heparanase was 96% in gastric carcinoma,was significantly higher than those in adjacent carcinoma and normal mucosa （P 〈 0. 01 ）. The high expression of heparanase in carcinoma was significantly related with the fibromembranous invasion （P 〈 0.05 ） ,TNM stage （ P 〈 0.01 ） and lymph node metastasis （ P 〈 0.01 ）. DI value was 1.26 ± 0. 14,1.05 ± 0. 14,1.00 ± 0.11 in carcinoma group, adjacent carcinoma group and normal group,There was a significant difference between them （P 〈 0.05 ）. The value of DI was significantly related with differential degree and lymph node metastasis（ P 〈 0.01 ）. There was positive correlation between value of DI and FI value of Hepara- nase （ r = 0. 536, P 〈 0.01 ）. Conclusions Heparanase may play an important role in the development of invasion and metastasis of gas- tric carcinoma. DNA content was related with malignant degree in carcinogenesis, heparanase and DNA content might used as an important index of the evaluation of malignant degree of gastric carcinoma and prognosis.

关 键 词：胃癌 乙酰肝素酶 DNA含量 流式细胞术 

分 类 号：R135.12[医药卫生—劳动卫生]