MDMA诱导大鼠神经元凋亡及凋亡相关因子caspase-3的表达  

Neuron apoptosis induced by 3,4-methylenedioxy methamphetamine and the expression of caspase-3

作　　者：王雪[1] 李静[1] 祝三平[1] 

机构地区：[1]四川大学华西医院心理卫生中心,四川成都610041

出　　处：《华西药学杂志》2007年第5期511-513,共3页West China Journal of Pharmaceutical Sciences

基　　金：四川大学华西医院基金资助(专项基金编号:134040162)

摘　　要：目的探讨3,4-亚甲基二氧基甲基苯丙胺(MDMA)对实验大鼠神经元凋亡的诱导及凋亡相关因子半胱氨酸天冬氨酸特异性蛋白酶-3(caspase-3)的表达。方法将20只Wistar雄性大鼠随机均分为1组对照组(A)、3组MDMA实验组(B、C、D)。B组予MDMA(20 mg.kg-1,ip,single),C组予MDMA(20 mg.kg-1,8 am,8 pm,ip×2 d),D组予MDMA(20 mg.kg-1,8 am,8 pm,ip×4 d);A组给予等体积生理盐水。采用TUNEL法检测神经元凋亡,免疫组织化学方法检测Caspase-3的表达。结果给予MDMA后,大鼠各相关脑区有凋亡细胞形成,Caspase-3有不同程度的表达。结论MDMA可导致神经元的凋亡,并诱导凋亡相关因子Caspase-3的表达。OBJECTIVE To explore the neuron apoptosis induced by 3,4 - methylenedioxy methamphetamine （MDMA） and the expression of Cysteinyl aspartate specific protease - 3 （ caspase - 3 ） , one protein related to apoptosis. METHODS 20 male Wistar rats were selected and randomly divided to into control group（A） and experimental groups（ B, C, D）, with 5 rats in each one. Rats in group B were injected with MDMA （20 mg ·kg^-1 , ip, single） , rats in group C were injected with MDMA （20 mg·kg^-1, 8 am 8 pm,ip ×2 d） ,rats in group D were injected with MDMA （20 mg·kg^-1 ,8 am 8 pm,ip ×4 d） ;rats in control group were administered with saline of same volume（ip）. Neuron apoptosis were measured by TUNEL, and the expression of caspase - 3 were detected in by immunohistochemistry. RESULTS Apoptosis neurons were detected in post - treatment of MDMA. Expression of the caspas - 3, relating to apoptosis, was observed at different level. CONCLUSION MDMA could cause neurons deficits because of neurons apoptosis, caspase - 3, the protein related to apoptosis, might be involved in the procress of apoptosis.

关 键 词：3 4-亚甲基二氧基甲基苯丙胺 神经元凋亡 半胱氨酸天冬氨酸特异性蛋白酶-3 表达 

分 类 号：R965[医药卫生—药理学]