氯化两面针碱体外诱导两种鼻咽癌株的细胞凋亡  

Effect of Nitidine chloride induced apoptosis in two nasopharyngeal carcinoma cell lines in vitro

作　　者：刘华钢[1] 秦三海[1] 王博龙[1] 杨斌[1] 

机构地区：[1]广西医科大学药理教研室,广西南宁530021

出　　处：《华西药学杂志》2007年第5期514-516,共3页West China Journal of Pharmaceutical Sciences

基　　金：广西科学研究与技术开发计划项目(桂科攻0630002-2E)

摘　　要：目的研究氯化两面针碱体外促进两种鼻咽癌细胞株凋亡的作用。方法应用MTT法检测细胞的增殖活性,DNA ladder检测、细胞形态学观察、酶联免疫法进行检测分析。结果氯化两面针碱对两种鼻咽癌7111,Ecv2细胞株的IC50分别为1.238±0.09、1.641±0.331μg.ml-1。在其作用下,两种鼻咽癌细胞株出现典型的凋亡特征,琼脂糖电泳出现典型的DNA梯带,细胞形态学表现出细胞核裂解、染色质聚集、核碎裂,胞浆浓缩、有空泡形成,ELISA法检测样本活性后发现药物作用后细胞中Caspase-3含量明显高于对照组(P<0.05)。结论氯化两面针碱有促进两种鼻咽癌7111,Ecv2细胞株凋亡的作用。OBJECTIVE To study Nitidine chloride on the inducing apoptosis effect in two nasopharyngeal carcinoma cell lines. METHODS M3T testing, DNA agarose gel electrophoresis, Cell morphology, ELISA were established on the carcinoma cells. RESULTS Treated with nitidine chloride, the IC50 of the two nasopharyngeal carcinoma cell lines 7111, Ecv2 were 1. 238 ± 0. 09, 1. 641 ± 0. 331 μg·ml^-1, and two nasopharyngeal carcinoma cell lines exhibited typical. Nuclear DNA of apoptosis cells displayed ladder bands characteristic of internucleosomal DNA fragmentation. Apoptosis cells demonstrated compaction and segregation of the nuclear chromatin, condensation and vacuolus of the cytoplasm. Examined by ELISA,the concentrations of caspase -3 in the groups with drug treated were higher than that of the control ones, and statistical significance was obtained （ P 〈 0. 05 ）. CONCLUSION Nitidine chloride can induce two nasopharyngeal carcinoma cell lines 7111, Ecv2 to apoptosis.

关 键 词：氯化两面针碱 凋亡 鼻咽癌 

分 类 号：R96[医药卫生—药理学]