乳化异氟醚和脂肪乳对大鼠心脏缺血再灌注损伤的影响  

Cadioprotective effects of emulsified isoflurane and intralipid on ischemia-reperfusion injury in isolated rat heart

作　　者：刘陕岭[1] 刘进[1] 

机构地区：[1]四川大学华西医院麻醉科,四川成都610041

出　　处：《华西药学杂志》2007年第5期525-527,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的研究乳化异氟醚和脂肪乳加入心脏保存液,对心脏复灌后的缺血再灌注损伤的影响。方法采用大鼠Langen-dorff心脏灌流及低温保存缺血再灌注损伤模型,测定乳化异氟醚和脂肪乳对心脏机械功能、冠脉流出液中磷酸肌酸激酶(CK)和乳酸脱氢酶(LDH)和心梗面积的影响。结果乳化异氟醚和脂肪乳均可显著增强左室发展压(LVDP),显著减少冠脉流出液中CK和LDH的含量,减少心梗面积。结论乳化异氟醚和脂肪乳加入心脏保存液能改善心脏机械功能,减少CK、LDH的释放,并缩小心梗面积。OBJECTIVE To investigate the cardioprotective effects of emulsified isofluane on ischemia - reperfusion injury after three hours cold storage. METHODS Isolated rat hearts were perfused with Krebs - Henseleit solution （KHS）and stored in four different cardioplegic solutions. After storage, hearts were reperfused for 2 hours with KHS. Left ventricular development pressure （LVDP） , maximum rate of pressure increase, minimum rate of pressure decrease （dp/dtmin） , coronary flow （CF） , Creatinine kinase （CK） , Lactate dehydrogenate （ LDH ） , and infarct size （ IS ） were measured individually. RESULTS The addition of emulsified isofiurane and intralipid to the preservation solution, improved the recovery of mechanical function （ P 〈 0. 05 ） , decreased the release of LDH and CK（P 〈0.05 ） and reduced the IS（P 〈0.05 ） of the heart during reperfusion, compared with ST- Ⅱ group. There was no siginigficant difference between El group, E5 group and Inralipid group with respects of LVDP % , dp/dtmax, myocardial enzyme activity and infarct size. CONCLUSION Emulsified isofiurane and Intralipid are beneficial to the donor hearts.

关 键 词：乳化异氟醚 脂肪乳 心脏保存 缺血-再灌注损伤 

分 类 号：R96[医药卫生—药理学]