延迟连续移植预防小鼠移植物抗宿主病的实验研究  

Experimental study on the prevention of mouse graft-versus-host disease by delayed sequential transplantaton

作　　者：王春玲[1] 徐开林[1] 潘秀英[1] 杜冰[1] 

机构地区：[1]徐州医学院附属医院血液科,江苏徐州221002

出　　处：《徐州医学院学报》2007年第10期631-635,共5页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金(30170389)

摘　　要：目的研究延迟连续移植对小鼠异基因移植后急性移植物抗宿主病（aGVHD）的影响。方法近交系BALB/c（H-2d）受鼠接受8.0 Gy全身放疗（TBI）后,分4组（每组n=20）输注供鼠C57BL/6（H-2b）骨髓和脾细胞的混合液：①经典移植组（TBI后4 h输注）;②连续移植组（TBI后4 h、1-3天连续输注）;③延迟移植组（TBI后4天输注）;④延迟连续移植组（TBI后4-7天连续输注）。流式细胞仪检测移植后各组T细胞亚群、NK细胞、H-2b的表达,比较各组受鼠生存率、aGVHD情况及造血重建。结果经典移植组均于3周内死于aGVHD,连续移植组、延迟移植组60天生存率分别是30%、50%。延迟连续移植组60天生存率为70%,高于其他3组（P＜0.05）。延迟连续移植组20天时外周血白细胞计数恢复正常,30天时T细胞亚群和NK细胞的表达恢复正常,60天时H-2b细胞的百分率为98.13%±1.11%。结论延迟连续骨髓移植可减轻小鼠异基因移植后的aGVHD,提高生存率,同时不影响骨髓植入、造血和免疫重建,是一种简单有效的预防aGVHD的新方法。Objective To study the effect of delayed sequential transplantation on acute graft - versus - host disease （aGVHD） after mouse allogeneic -bone marrow transplantation （allo - BMT）. Methods C57BL/6 （H -2^b） mice were used as donors and BALB/c （H -2d） mice were used as recipients. The BALB/c mice, divided into four groups （ n =20 each）, were given 8.0 Gy total body irradiation （TBI） on day 0 and the infusion Of a blend of bone marrow cells and spleen cells in four regimes respectively, i. e. ： transplantation 4 h after TBI （known as classic transplantation） in group 1, transplantation from 4 h through day 3 after TBI （ sequential transplantation） in group 2, transplantation on day 4 after TBI （ delayed transplantation） in group 3, and transplantation from day 4 through day 7 after TBI （ delayed sequential transplantation）. The levels of recipient＇s H -2b cells, subpopulations of T lymphocyte and NK cell were detected by flow cytometry. The survival rate, clinical manifestations of aGVHD and hematopoietic recovery in the recipient mice were observed. Results The mice in group 1 all died of aGVHD within 3 weeks after transplantation. The survival rates in groups 2 and 3 were 30% and 50% respectively on day 60. The survival rate in group 4 was 70% on day 60, which was higher than those in the other three groups （ P 〈 0.05 ）. The mean value of donor - derived cell （ H - 2b cells） in group 4 was 98.13% ± 1.11% on day 60, in which the WBC count reached the normal value on day 20; the subpopulations of T lymphocyte and NK cell reached their normal values on day 30. Conclusions The delayed sequential transplantation is a simple and effective new way to prevent aGVHD after allo - BMT, improving the survival rate and bringing no bad effects onto the engraftment and the reconstitution of hematopoiesis and immunity in the recipient mice.

关 键 词：骨髓移植 延迟连续移植 移植物抗宿主病 

分 类 号：R457.7[医药卫生—治疗学]