叶下珠有效部位总提取物致突变实验研究  

Study on Mutagenicity of Total Extracts of Yexiazhu(TEY)

作　　者：曹毓[1] 刘玉清[2] 温志坚[1] 唐云安[2] 

机构地区：[1]四川省中药研究所,四川成都610041 [2]华西医科大学,四川成都610041

出　　处：《时珍国医国药》2007年第10期2475-2477,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的评价叶下珠有效部位总提取物的致突变性。方法采用微核实验、Ames实验和体外染色体畸变实验对叶下珠有效部位总提取物进行了诱变性检测。结果小鼠体内骨髓细胞微核实验未发现骨髓细胞微核率增加,Ames实验中对TA97,TA98,TA100,TA102 4种菌株加与不加S9均未发现回变菌落数增加,体外染色体畸变实验加与不加S9均未发现染色体畸变率超过5%。3个实验结果均为阴性。结论在该实验条件下,未检出叶下珠有效部位总提取物的诱变性。Objective To evaluate the mutagenicity of total extracts of Yexiazhu （TEY）. Methods The micronucleus test in mice, Ames test and chromosome aberration test were carried out. Results The micronucleus rates in all doses were not significandy different from the control group. No increase in the number of revertant colonies was found in the Ames test with and without S9 activation system. Negative results were seen from chromosome aberration test in CHL cell. Conclusion No mutagenicity effects of TEY were detected in this study .

关 键 词：叶下珠有效部位总提取物 诱变性 微核实验小鼠体内骨髓细胞微核实验 AMES实验 体外染色体畸变实验 

分 类 号：R285.5[医药卫生—中药学]