补肾益精颗粒提取工艺的研究  

Optimization of Extracting Process for Bushenyijing Granules by Orthogonal Test

作　　者：吴月国[1] 张萍[1] 孙洁胤[1] 马丽萍[2] 刘骅[1] 

机构地区：[1]浙江省医学科学院药物所,浙江杭州310013 [2]上海交通大学药学院,上海200240

出　　处：《时珍国医国药》2007年第10期2506-2507,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的优化补肾益精颗粒的提取制备工艺。方法以淫羊藿苷的含量为指标,通过渗漉实验和正交设计回流实验筛选最佳提取条件。结果渗漉提取的最佳工艺为用70%乙醇作溶剂,浸渍48 h后,以1-3 ml/min的速度缓缓渗漉。正交设计实验最佳回流工艺为：用8倍量50%乙醇,提取1次3 h。结论正交设计回流实验优选得到的淫羊藿乙醇回流法的提取工艺条件稳定可行。Objective To optimize the extracting process for Bushenyijing Granules. Methods With content of icariin as the inspection indicators, percolation method and orthogonal test were designed for optimization of extracting process for Bushenyijing Granules. Orthogonal test was used to investigate the influence of four extracting factors （concentration of alcohol （A）, alcohol volume （B）, extracting time （C） and times of extracting （D） ） on the extracting process. Results The optimum percolation method was that the medicinal material was percolated at 1 to 3 ml/min after soaked for 48 hours in 70% alcohol. The optimum extracting process was A2 DI C3B2, i.e. the 50% alcohol volume being 8 times of the weight of medicinal material, extraction for 1 times and 3 hours each time. Conclusion Optimization of extracting process for Bushenyijing Granules by orthogonal test is a simple, quick and accurate method.

关 键 词：补肾益精颗粒 淫羊藿苷 渗漉法 正交实验 

分 类 号：R284.2[医药卫生—中药学]