山莨菪碱对妊娠期高血压疾病患者腹腔巨噬细胞分泌细胞因子的影响  

Effect of anisodamine on cytokine secretion of peritoneal macrophages in patients hypertensive disorder of Pregnancy

作　　者：赵彩珍[1] 乔福元[2] 

机构地区：[1]武警湖北总队医院妇产科,武汉430061 [2]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《武警医学》2007年第10期740-743,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的观察山莨菪碱(654-2)对妊娠期高血压疾病患者腹腔巨噬细胞分泌肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的影响,探讨其对妊娠期高血压疾病治疗作用的免疫机制。方法分离、提纯妊娠期高血压疾病患者腹腔巨噬细胞后,分别用RPMI1640或含0.5mg/ml654-2的RPMI1640培养液培养24h,应用ELISA方法和RT-PCR方法,分别检测巨噬细胞培养上清液中TNF-α、IL-6水平及两者mRNA表达情况。结果(1)654-2可明显降低妊娠期高血压疾病各组巨噬细胞培养上清液中TNF-α水平(P<0.05或P<0.01)。(2)654-2可明显降低MP组、SP组巨噬细胞培养上清液中IL-6水平(P<0.05或P<0.01)。(3)654-2可明显降低妊娠期高血压疾病各组巨噬细胞TNFαmRNA表达量(P<0.05或P<0.01)。(4)654-2可明显降低MP组、SP组巨噬细胞TNFαmRNA表达量(P<0.05或P<0.01)。结论654-2对妊娠期高血压疾病患者不仅具有解痉作用,同时对腹腔巨噬细胞过度活化具有显著抑制作用,可在转录和翻译两个水平抑制TNF-α、IL-6产生,在一定程度上防止妊娠期高血压疾病的发生和发展。Objective To the immunomechanism of anisodamine （654- 2）in treatment of hypertensive disorder of prognancy（HDOP）, and its effect on TNF -α and IL - 6 levels secreted by peritoneal maerophages. Methods The isolated peritoneal macrophages from pregnant women with HDOP were cultured for 24 hours in RPMI 1 640 medium supplemented with 10% fetal bovine serum or in RPMI 1 640 with 5 mg/mL anisedamine and 10% fetal bovine serum; the TNF -α and IL - 6 levels and their mRNA expression were detected by ELISA and RT - PCR, respectively, Results （1） Anisedamine significantly reduced TNF - α level at GH group （ P 〈 0.05 ）, MP group （ P 〈0.01） and SP group （ P 〈 0.01）.（2）Anisedamine significantly reduced IL-6 level in MP group （ P 〈0.05 ） and SP group （ P 〈 0.01）. （3）Anisedamine significantly reduced TNF-α mRNA expression in GH group （ P 〈 0.05 ）, MP group （ P 〈 0.01 ） and SP group （ P 〈 0.01 ）. （4）Anisedamine significantly reduced IL - 6 mRNA expression in MP group （ P 〈 0.05 ） and SP group （ P 〈 0.05）. Conclusions Anisedamine has spasmolytic effect on HDOP, inhibits the excessive activation of peritoneal macrophages, inhibits TNF - α and IL - 6 production at both transcription and translation levels, and prevents the occurrence and progression of I-IDOP.

关 键 词：妊娠期高血压疾病 巨噬细胞 肿瘤坏死因子-α 白细胞介素-6 腹腔 山莨菪碱 

分 类 号：R714.246[医药卫生—妇产科学]