柳蚕卵黄原蛋白cDNA的克隆及序列分析  

Clone and Analysis of cDNA of Vitellogenin from Actias selene Hubner

作　　者：尹志亮[1] 刘朝良[1] 邵引刚[1] 李锋[1] 魏国清[1] 穆莉[1] 孙辉[1] 钱岑[1] 

机构地区：[1]安徽农业大学生命科学院特种经济动物科学系,安徽合肥230036

出　　处：《激光生物学报》2007年第5期552-558,共7页Acta Laser Biology Sinica

基　　金：国家自然科学基金资助项目(30371088)

摘　　要：经RT-PCR和RACE合成了柳蚕卵黄原蛋白(Vg)cDNA,经序列分析其长度为5701个碱基,由一个ORF组成,编码了1 774个氨基酸。氨基酸序列中有2个保守的多聚丝氨酸区域。并推测氨基酸序列中有8个天冬酰胺连接的糖基化位点(N-linked glycosylation sites)。与樟蚕、天蚕、柞蚕、野桑蚕、家蚕、樗蚕、蓖麻蚕的VgcDNA序列比对后发现有很高的同源性,分别为83.1%、81.1%、81.0%、64.7%、64.6%、79.7%、79.2%,同其它昆虫Vg cDNA序列也具有很高的同源性。根据7种蚕(家蚕、天蚕、柞蚕、蓖麻蚕、樟蚕、野桑蚕、樗蚕)VgcDNA序列建立了系统发生树。The eDNA sequence of vitellogenin in Actias selene Hubner is obtained through RT-PCR to the total RNA and RACE amplification to 3＇-end, which contains 5 701 bases, an opening reading frame which encodes 1 774 amino acids, composed of 8 N-linked glycosylation sites ,2 polyseme regions, Homology of bases between Actias selene Hubner, Saturnia japonica（ AB190809）, Antheraea yamamai（ AB055843 ）, Antheraea pernyi（ AB049631 ）, Bombyx mandarina （ AB055845 ）, Bombyx mori（ D13160 ）, Samia cynthia pryeri （ AB190810 ）, Philosamia cynthia ricini（ ab0555844 ）. Homology identities are 83.1% ,81.1% ,81.0 % ,64.7 % ,64.6 % ,79.7 % ,79.2 % respectively. Homology identity is high compared with other insects. A phylogenetic tree was established based on the vitellogenin eDNA of seven species, including Antheraea pernyi , Antheraea yamamai , Bombyx mori , Philosamia cynthia ricini , Bombyx mandarina , Samia cynthia pryeri ,Saturnia japonica.

关 键 词：柳蚕 卵黄原蛋白 CDNA克隆 序列分析 

分 类 号：Q2-33[生物学—细胞生物学] S88[农业科学—特种经济动物饲养]