[^188Re（CO）3（H2O）3]^＋间接标记免疫磁性纳米微粒的实验研究  

Labeling of immuno-magnetic nanoparticle with [^188Re（CO）3（H2O）3]^＋

作　　者：冯彦林[1] 谭家驹[1] 梁生[2] 孙静[1] 吴校连[1] 司建华[1] 夏姣云[2] 温广华[1] 

机构地区：[1]中山大学附属佛山医院（佛山市第一人民医院）核医学科,广东佛山528000 [2]中国科学院应用物理研究所,上海200000

出　　处：《国际放射医学核医学杂志》2007年第4期211-214,共4页International Journal of Radiation Medicine and Nuclear Medicine

摘　　要：目的探讨标记单克隆抗体磁性纳米微粒的实验条件。方法以[二（2-吡啶甲基）-氨基]-乙酸（PADA）作为双功能螯合剂，将[^188Re（CO）3（H2O）3]^＋间接标记到耦联了单克隆抗体的磁性纳米微粒。结果[^188Re（CO）3（H2O）3]^＋间接标记免疫磁性纳米微粒的标记率大于80％，在小牛血清和生理盐水中48h后稳定性仍能保持在90％以上。结论使用PADA作为双功能螯合剂，[^188Re（CO）3（H2O）3]^＋间接标记免疫磁性纳米微粒的标记率高，稳定性好，适于进一步体内研究。Objective To investigate a fine experiment condition of indirect labeling method that Hepama-1-SPIONs（superparamagnetic iron oxide nanoparticles）was labelled with rhenium-188 complex （[^188Re（CO）3（H2O）3]^＋）. Methods TheHepama-1-SPIONsbridgedbybifunctionalchelatingagent2-picolylamine- N,N-diacetic acid （PADA）was labelled rhenium-188-tricarbonyl complex prepared with high labeling yields. Results The labeling rate of [^188Re（CO）3（H2O）3]^＋ -PADA- Hepama-l-SPIONs was more than 80%, and was extremely stable in vitro, The labeling rate of [^188Re（CO）3（H2O）3] -PADA- Hepama-l-SPIONs was more than 90% after48 in calf serum and isotonic Na chloride. Conclusions ^188Re（CO）3（H2O）3- PADA- Hepama- 1- SPIONs shows it＇s fine stability and biological properties , and it is well worth further researching as bio-magnetically targeted radiotherapy for cancer.

关 键 词：同位素标记 铼 螯合剂 磁性纳米微粒 

分 类 号：TB38[一般工业技术—材料科学与工程]