比色法测定大麻药及其提取物中总皂苷的含量  

Determination content of total Saponins of dolichos tenuicaulis(Baker) Craib and crude extracts by spectrophotometry

作　　者：许丽娜[1] 蔡博能[1] 尹连红[1] 王晓娜[1] 彭金咏[1] 

机构地区：[1]大连医科大学药学院,辽宁大连116044

出　　处：《大连医科大学学报》2007年第5期448-450,共3页Journal of Dalian Medical University

摘　　要：[目的]建立大麻药及其相关提取物的总皂苷含量分析方法。[方法]样品经香草醛-高氯酸显色后,以人参皂苷Rg1为对照品,进行紫外分光光度测定,优选最佳分析条件。[结果]最佳分析条件为：0.2 mL 5%香草醛-冰醋酸溶液和0.8 mL高氯酸为显色剂,显色温度为60℃,显色时间为15 min,测定波长为550 nm。样品在3.25-19.52μg/mL范围内呈良好的线性关系,回归方程为Y=0.030X＋0.117（r=0.9995）,平均加样回收率为97.45%（RSD=1.75%,n=5）。[结论]本法可作为大麻药及相关提取物中皂苷的质量控制方法。[ Objective] To establish a method for determination of total saponins of dolichos tenuicaulis （Baker） Craib and crude extracts by UV. [ Methods ] The samples were determined by UV after reaction with vanillin -perchloric acid, and ginsenoside Rg1 was selected as the standard. Some parameters which might effect the results were all investigated and optimized. [ Results ] The suitable analytic conditions were optimized as follows： 5% vanillin - acetic acid solution 0.2 mL, perchloric acid 0.8 mL, temperature 60℃, reaction time 15 min. ,detection wavelength 550 nm. The samples were linear in the range of 3.25 - 19.52 μg/mL with the regression equation of Y = 0. 030X ＋ 0. 117 （ r = 0.9995 ）, and the average recovery was 97.45% （ RSD = 1.75 %, n = 5 ） under the selected conditions. [ Conclusion] This method is suitable for the determination of total saponins for quality control of dolichos tenuicaulis （Baker） Craib.

关 键 词：大麻药 总皂苷 比色法 

分 类 号：R284[医药卫生—中药学]