米诺环素对大鼠肾缺血再灌注损伤的保护作用  

Protective effects of minocycline on renal ischemia-reperfusion injury in rats

作　　者：余晓东[1] 吴小候[1] 杜孝文[1] 李俊[1] 

机构地区：[1]重庆医科大学附属第一医院泌尿外科,重庆400016

出　　处：《重庆医科大学学报》2007年第11期1172-1174,1214,共4页Journal of Chongqing Medical University

摘　　要：目的:探讨米诺环素对大鼠肾缺血再灌注损伤的保护作用及机制。方法:72只SD雄性大鼠随机分成3组:假手术组(Sham组),缺血再灌注组(Ir组),米诺环素组(M组),每组各4个时相(6h、12h、24h、72h)。采用切除右肾,夹闭左肾动脉45min后恢复灌流建立缺血再灌注损伤模型。测定髓过氧化物酶(MPO)活性及血清肌酐(Scr)水平,以反映中性粒细胞活化及肾功能损害的程度。观察肾组织病理学改变及超微结构变化。结果:缺血再灌注组MPO活性和Scr水平升高,米诺环素组明显低于缺血再灌注组,2组比较差异有显著性意义(P<0.05,P<0.01)。缺血再灌注组出现明显的肾组织病理学及超微结构损害,米诺环素组上述变化轻于缺血再灌注组。结论:米诺环素对大鼠肾缺血再灌注损伤具有保护作用。Objective：To investigate the protective effects of minocycline on renal ischemia-reperfusion injury and its mechanism in rats. Methods：72 male Sprague-Dawley rats were randomly divided into three groups： sham-operated （Sham）, ischemia-reperfusion （Ir）,minocycline hydrochloride （M）,for each of the four phases （6h.12h.24h,72h）. 45 minutes after the left renal artery occlusion on the basis of the right renal resection,we restored perfusion under modeling of ischemia-reperfusion injury.Determined myeloperoxidase （MPO） activity and serum creatinine （Scr）,which indicated the extent of the injury for neutrophil infiltration and renal function.Observed renal histological and ultrastructural changes.Results：The level of MPO and Scr was elevated in Ir,and it was distinctly lower in M than that in Ir.Difference between the two groups was of great significance （P〈0.05,P〈0.01）.Pathological changes and uhrastructural lesion in renal tissue was identified morphologically and structurally after reperfusion,and significantly relieved in M. Conclusion： Minocycline has protective effects on renal ischemia-reperfusion injury in rats.

关 键 词：米诺环素 缺血再灌注损伤 超微结构 保护作用 

分 类 号：R965[医药卫生—药理学] R691.5[医药卫生—药学]