RP-HPLC测定返魂草颗粒中绿原酸和咖啡酸含量  

Determination of chlorogenic acid and caffeic acid in Fanhuncao Granule by RP-HPLC

作　　者：刘艳清[1] 汪洪武[1] 鲁湘鄂[1] 

机构地区：[1]肇庆学院轻工化学系,广东肇庆526061

出　　处：《中成药》2007年第10期1451-1453,共3页Chinese Traditional Patent Medicine

基　　金：肇庆市科技创新项目(2006G21);肇庆学院科研基金资助项目(0643)

摘　　要：目的：建立返魂草颗粒（返魂草）绿原酸和咖啡酸的反相高效液相色谱分析方法。方法：Eclipse XDB-C18柱,乙腈-0.4%磷酸（12∶88,V/V）为流动相,柱温：室温,于327 nm测定返魂草颗粒中的绿原酸和咖啡酸。结果：绿原酸在0.005 6~0.896μg/L,咖啡酸在0.012 5~0.1μg/L内线性关系良好,平均回收率分别为100.1%、99.8%。结论：该法简便、准确、无干扰,可用于测定返魂草颗粒中绿原酸和咖啡酸含量。AIM： To develope a reversed-phase high performance liquid chromatographic method for determing chlorogenic acid and caffeic acid in Fanhuncao Granule （Senecio cannabifolius Less）. METHODS： The operation was carried out on Eclipse XDB-C18 column with the mobile phase consisting of a mixture of acetonitrile-0.4% phosphoric acid （ 12 ： 88, V/V）. The flow rate of 1.0 mL/min and UV detection wavelength at 327 nm were set to determine the contents of chlorogenic acid and caffieic acid. RESULTS： There was good linear relationship between the concentrations and the peak-areas of chlorogenic acid and caffeic acid. The two kinds of standard solutions were both stable in 16 h （RSD = 1.55% for chlorogenic acid, 1.11% for caffeic acid） The average recovery was 100. 1% and 99.8% for chlorogenic acid and caffeic acid, repectively. CONCLUSION： The method is simple, sensitive, rapid and accurate, and can be used for the quality control of Fanhuncao Granule.

关 键 词：反相高效液相色谱法 返魂草颗粒 绿原酸 咖啡酸 

分 类 号：R927.2[医药卫生—药学]