乙型肝炎病毒宫内感染与IFN-γ和IL-4、IL-6细胞因子的相关性  

Study on the relationship between IFN-γ,IL-4 and IL-6 in HBsAg positive pregnant women to intrauterine infection of hepatitis B virus

作　　者：梅燕 黄星[2] 

机构地区：[1]广西柳州市妇幼保健院,545001 [2]广西医科大学第一附属医院妇产科,南宁530021

出　　处：《中国优生与遗传杂志》2007年第10期37-38,106,共3页Chinese Journal of Birth Health & Heredity

基　　金：广西科学基金项目(NO:0339027)

摘　　要：目的研究乙型肝炎病毒宫内感染与白细胞介素-4(IL-4)、白细胞介素-6(IL-6)和干扰素-γ(IFN-γ)细胞因子的相关性。方法将研究对象分为两组:研究组为80例乙型肝炎病毒表面抗原(HBsAg)阳性孕妇;对照组为20例正常孕妇。采用双抗夹心酶联免疫吸附法(DAS-ELISA)检测孕妇外周静脉血及其新生儿脐静脉血血清中乙型肝炎五项指标及细胞因子IFN-γ、IL-4、IL-6水平。结果研究组孕妇分娩的新生儿80例有11例宫内感染,宫内感染率为13.75%。新生儿HBV宫内感染组孕妇血清中IFN-γ水平显著低于HBV宫内未感染组及对照组孕妇(P<0.01),IL-4、IL-6水平则显著高于HBV宫内未感染组及对照组孕妇均有统计学意义(P<0.01)。HBV宫内未感染组与对照组相比,上述三种细胞因子水平差异均无统计学意义(P>0.05)。上述各组孕妇血清中IL-4与IL-6水平均呈显著正相关(P<0.01,P<0.01,P<0.01);IFN-γ与IL-4呈显著负相关(P<0.01,P<0.01,P<0.01);IFN-γ与IL-6亦呈显著负相关(P<0.01,P<0.01,P<0.01)。各组新生儿脐血清中IFN-γ、IL-4、IL-6水平差异无统计学意义(P>0.05)。结论孕妇细胞免疫功能紊乱导致IFN-γ抗病毒作用减弱,IL-4、IL-6水平升高,不利于孕妇体内HBV清除,易导致胎儿宫内感染。Objective： To study on the relationship between intrauterine infection of hepatitis B virus and IL- 4, IL- 6 and IFN -γcytokines. Methods ： The pregnant women were divided into two groups ： 80 cases with positive HBsAg were included in the study group, the control group included 20 cases of normal pregnant women. By using diantibody sandwich enzyme linked immuneosorbant assay （DAS- ELISA）, the serum HBVM, IFN -γ, IL-4, and IL- 6 levels in maternal and neonates were determined respectively. Results： In the study group, the incidence of HBV intrauterine infection was 13.75% （11/80）. The IFN-γlevels of the pregnant women with intrauterine infection were significantly lower than those without intrauterine infection and control group （ P 〈 0. 01 ）, IL - 4, IL - 6 levels was contrary to IFN -γ （ P 〈 0. 01 ）. There had no significant difference of IFN -γ, IL - 4, IL - 6 levels between women without intrauterine infection and control group （ P 〉 0. 05 ） . The positive correlation existed between IL- 4and IL- 6 levels among each group （ P 〈 0. 01 ）, IFN -γand IL - 4 levels were negatively correlated, and so were IFN -γand IL - 6 levels （ P 〈0. 01 ）, No significant difference of IFN -γ, IL- 4, IL- 6 levels as found among in newborns group （ P 〉 0. 05）. Conclusion： There is imbalance of TH1/TH2 cells in pregnant women, which causes IFN -γactivities decrease, and the activity of IL -4, IL - 6 increases. Thus the antiviral responses of IFN -γare inhibited, resulting higher incidence of intrauterine infection.

关 键 词：乙型肝炎病毒 宫内感染 细胞因子 IL-4 IL-6 IFN-Γ 

分 类 号：R714.2[医药卫生—妇产科学]