3-硝基丙酸预处理保护多巴胺神经元机制研究  

Neuroprotection of 3-NPA Preconditioning on Dopaminergic Neuron

作　　者：梁直厚[1] 邓学军[1] 孙圣刚[1] 曹学兵[1] 李红戈[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经科,武汉430022

出　　处：《医药导报》2007年第11期1255-1258,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(基金编号:30170334)

摘　　要：目的研究3-硝基丙酸(3-NPA)多次预处理保护多巴胺神经元的作用及其可能机制。方法使用MPP+在分泌多巴胺的人神经纤维瘤细胞SH-SY5Y上制作帕金森病细胞模型,在模型制作前分别1或5次加入3-NPA(0.2 mmol.L-1)进行预处理,分别应用液闪烁仪检测[3H]DA摄取率,双波长分光光度仪检测细胞内游离钙离子(Ca2+)浓度的改变,观察3-NPA预处理保护多巴胺神经元的作用。结果MPP+可降低[3H]DA摄取率,升高细胞内游离钙离子浓度,3-NPA预处理可提高[3H]DA摄取率,降低胞内游离钙离子浓度,3-NPA多次预处理较3-NPA单次预处理的作用更好。结论3-NPA预处理对多巴胺神经元有明显的保护作用,可能与其降低胞内游离钙离子浓度有关。Objective To investigate the protective effect of 3-NPA repetitive preconditioning on dopaminegic neuron, Methods The Parkinson＇s disease model was set up by using MPP^＋ on the neuroblastoma SH-SYSY which secreting dopamines. Before incubation of MPP^＋ , 3-NPA （0.2mmol·L^-1） was added into the cell for once or 5 times. The ratio of uptaking [^3H] DA and intracellular free calcium were detected. Results The ratio of uptaking [^3H] was decreased and the intracellular calcium was increased by MPP^＋, Repetitive preconditioning of 3-NPA showed better effects than that of single preconditioning, Conclusion Preconditioning of 3-NPA strongly protects dopaminegic neuron, the mechanism of which may be associated with reducing the intracellular calcium.

关 键 词：3-硝基丙酸 帕金森病 预处理 神经保护 

分 类 号：R971[医药卫生—药品] R965[医药卫生—药学]