卡莫氟固脂纳米粒的制备及其体外逆转人大肠癌细胞多药耐药性研究  

Preparation of HCFU-SLNs and Reversion of Multidrug Resistance to Human Carcinoma Line in vitro

作　　者：孟胜男[1] 赵妍[1] 王欣[1] 王怀良[2] 

机构地区：[1]中国医科大学药学院药剂教研室,沈阳110001 [2]中国医科大学药学院临床药理教研室,沈阳110001

出　　处：《医药导报》2007年第11期1261-1263,共3页Herald of Medicine

基　　金：辽宁省教育厅科学研究计划资助项目(基金编号:05L472)

摘　　要：目的研制卡莫氟固脂纳米粒(HCFU-SLN)并检测其相关参数,考察其对大肠癌细胞(CCL-187)多药耐药的逆转作用。方法采用溶剂乳化法制备HCFU-SLN,透射电镜观察其外部特征,激光粒度仪测定其粒径和粒径分布,MTT法考察其对CCL-187多药耐药的逆转作用。结果HCFU-SLN外观圆整,平均粒经为(172.9±70.5)nm,包封率为92.0%。其对敏感细胞的细胞毒作用与游离卡莫氟的作用差异无显著性,对耐药细胞的杀伤力较游离卡莫氟显著增强(P<0.05),CCL-187对HCFU-SLN的敏感性是对游离卡莫氟的5.5倍。结论卡莫氟固脂纳米粒可有效逆转人大肠癌细胞多药耐药。Objective To study the preparation muhidrug resistance to human carcinoma cell lines in vitro. and characteristics of HCFU-SLN and explore the reversion of Methods HCFU-SLN were preparaed by solvent emulsification. The partical size and appearance of HCFU-SLN were measured by laser parfical size instrument and electron transmission microphotography, reversion of MDR was determined hy MTT assay. Results The appearance of particles was intact and round, the average diameter of HCFU-SLN was （172.9±70.5 ）nm and the encapsulating rate was 92%, In the sensitive cell lines, the HCFU-SLN and free HCFU showed the same cytotoxic effect; but for the resistant cells, HCFU-SLN were more effective than that of free HCFU（P 〈0.05 ） ; LLC-187/HCFU was 5.5 times more sensitive to HCFU-SLN than to free HCFU. Condusion HCFU-SLN can effectively reverse the MDR to CCL-187 lines.

关 键 词：卡莫氟固脂纳米粒 多药耐药 逆转 

分 类 号：R915[医药卫生—微生物与生化药学] R979.14[医药卫生—药学]