小檗碱对糖尿病模型大鼠血清血小板糖蛋白GPVI表达的影响  

Effects of Berberine on the Expression of GPVI on Platelets of Diabetes Rats

作　　者：陆雷[1] 张怀勤[2] 陈国荣[2] 吴建波[2] 陈智理[1] 周雪英[1] 姜昌浩[1] 

机构地区：[1]浙江省义乌市中心医院,322000 [2]温州医学院第一附属医院,325000

出　　处：《医药导报》2007年第11期1287-1291,共5页Herald of Medicine

摘　　要：目的探讨血小板糖蛋白Ⅵ在糖尿病模型大鼠的表达情况,以及小檗碱对其影响。方法将雄性SD大鼠40只随机分成正常对照组、高脂组、糖尿病模型组和小檗碱治疗组,每组10只。正常组大鼠喂以普通饲料,其余30只大鼠喂养高脂饲料。8周后糖尿病模型组和小檗碱治疗组采用STZ制作糖尿病模型,正常组和高脂组给予相同剂量0.1 mol.L-1pH值为4.5的柠檬酸缓冲液腹腔注射。两周后定期剪尾测血糖,血糖>7.8 mmol.L-1者表示糖尿病模型制作成功。继续原饮食,小檗碱治疗组待模型制作成功后灌胃给予20.25 g.L-1小檗碱,100 mg.kg-1.d-1。其余3组灌胃给予等体积纯化水,持续8周后处死各组大鼠,观察各预定指标。结果流式细胞仪测定结果:糖尿病模型组大鼠GPⅥ、GPⅡb、CD40L表达均高于正常组(均P<0.05)。以上指标小檗碱治疗组与糖尿病模型组差异无显著性。GPⅥ与血糖值、CD41、CD40L均呈正相关。扫描电子显微镜显示,糖尿病模型组大鼠主动脉内皮正常纹理消失,粘附较多血小板,较多网状纤维和胶原暴露。小檗碱治疗组主动脉内皮有较多血小板聚集,粘附在内皮上,与内皮粘连紧密。结论糖尿病大鼠血小板GPⅥ表达明显增加,血小板活化,血小板在动脉内皮的粘附增加诱导内皮炎症加剧和血栓形成。GPⅥ与糖尿病组大鼠的动脉粥样硬化和血栓形成有关。小檗碱治疗能抑制GPⅥ的表达,但差异无显著性,可能与样本较小有关。Objective To investigate the expression of GPVI on platelets of diabetes rats induced by STZ and effects of berberine on it. Methods Forty male rats were randomly divided into four groups：control group, fat enriched diet group, diabetes group, diabetes rats treated with berberine, 10 rats in each group. Except for noraml rats, 30 others were fed with lipid enriched food. After fed with fat enriched diet for 8 weeks, the rats in diabetic groups were i.p. injected with 1% STZ, others were i.p. injected with the same volume of citrate buffer vehicles. After successful establishment of diabetic model two weeks later, one group of rats was treated with berberine at the dose of 100 mg·kg^-1· d^-1 for 8 weeks. Results Expression of GPVI（CD32） ,GP II b（ CD41 ） ,CD40L（ CD154 ） was higher in the diabetic group than that in normal control group（ P 〈 0.05 for all） by FCM analysis. And no significant difference in all above expressions was found between berberine therapeutic and diabetic model group. There was a direct correlation between GPVI and blood glucose, CD41, CD40L. Scanning electron microscope indicated that the normal lamination of artery endothelial cell disappeared in diabetic models, accompanied with many platelets adhesion,and much netted fibres and collagen exposed. In the berberine treatment group, a few platelets aggregated to artery endothelials. Conclusion The expression of GPVI on platelets of diabetic rats was siginificanfly increased and platelets were activated, which induced inflammation of endothelial tissues and the formation of thrombus. GPVI may contribute to artherosclerosis and thrombus formation in diabetic rats. Berberine could inhibit the expression of GPVI, but couldn＇t reach to the statistics difference, which may be due to small samples.

关 键 词：小檗碱 GPVI CD40L 糖尿病 动脉粥样硬化 血小板 血管内皮 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]