氧化苦参碱对小鼠树突状细胞成熟和功能的影响  

Effect of oxymatrine on maturation and function of murine bone marrow derived dendritic cells

作　　者：张维[1] 周伯平[2] 陈心春[2] 袁静[2] 李美忠[2] 

机构地区：[1]湖北中医学院中医系,湖北武汉430061 [2]深圳市东湖医院

出　　处：《中西医结合肝病杂志》2007年第5期290-292,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:研究氧化苦参碱(OXY)对小鼠树突状细胞(DC)成熟、表型及功能的影响。方法:流式细胞术检测DC表面分子CD40的表达;混合淋巴细胞反应(MLR)检测DC对T淋巴细胞的刺激能力;ELISA法检测MLR上清中细胞因子IFN-γ的分泌。结果:第0天OXY处理组较对照组DC表面分子CD40的表达明显升高(P<0.01),刺激T细胞能力增强,分泌细胞因子IFN-γ明显升高(P<0.05),对LPS诱导的DC成熟,与DC+LPS组对照显著升高(P<0.05)。结果:OXY对DC的成熟和功能有一定的促进作用。Objective： To investigate the effects of oxymatrine on maturation and funtction of murine bone marrow de- rived dendritic cells in vitro. Methods： Bone marrow derived dendritic cells were treated with oxymatrine at day 0 and 5. DC maturation was induced by LPS at day 5. The phenotype of DC was determined by flow cytometry. The effect of oxymatrine on immuno-stimulatory capability of DCs was determined by [^3H] Thymidine incorporation assay. ELISA was used to measure the concentration of interferon-γ（IFN-γ） in supernatants of cell cultures. Results： The expression of CD40 on DCs treated with oxymatrine was higher than the control DCs group （P〈0.01）. IFN-7 production and cellular proliferation of splenocytes stimulated by oxymatrine treated DCs were higher than the control DCs group （P〈0.05）. Conclusion： Oxymatrine can enhance the maturation and function of murine bone marrow derived dendritic cells.

关 键 词：氧化苦参碱 树突状细胞 免疫功能 

分 类 号：R285.5[医药卫生—中药学]