李氏禾种子生活力的四唑法测定研究  

Study on the Testing of Seed Viability of Leersia hexandra with Triphenyl Ttetrazolium Chloride Method

作　　者：朱桂才[1] 姚振[2] 罗春梅[2] 周文林[2] 杨中艺[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]长江大学园艺园林学院,湖北荆州434025

出　　处：《长江大学学报（自科版）（中旬）》2007年第3期40-41,50,共3页Journal of Yangtze University(Nature Science Edition)

基　　金：湖北省教育厅青年项目(Q200512005)

摘　　要：比较了李氏禾(Leersia hexandra)种子在25℃、30℃和35℃3种温度下分别用0.5%和1.0%的TTC溶液染色的效果,以探讨李氏禾种子生活力的四唑法测定条件。结果表明:在35℃、0.5%的TTC浓度下染色30h可获得最佳染色效果,可作为李氏禾种子生活力测定的适宜条件。In order to research the conditions of determining seed viability of Leersia hexandra with TTC(triphenyl tetrazolium chloride) method,the seed viability of Leersia hexandra was determined under the conditions of different temperature(25 ℃,30 ℃ and 35 ℃) and TTC concentration(0.5% and 1.0%) during the period of different dyeing time(0~48 h).The results showed that under the conditions of the temperature of 35 ℃,the TTC concentration of 0.5% and at the dyeing time of 30 h,the dyeing effect was best.They could be the most appropriate condition to determine Leersia hexandra seed viability.

关 键 词：李氏禾(Leersia hexandra) 种子生活力 四唑 

分 类 号：Q945.3[生物学—植物学] Q949.714.2