白细胞介素-18在早期心肌缺血和心肌梗死心肌的表达  

An immunohistochemical study on IL-18 in early myocardial ischemia and myocardial infarction

作　　者：刘云[1] 邓世雄[2] 李剑波[2] 

机构地区：[1]川北医学院法医学系,四川南充637000 [2]重庆医科大学法医学教研室,重庆400016

出　　处：《中国法医学杂志》2007年第4期244-246,I0005,共4页Chinese Journal of Forensic Medicine

摘　　要：目的探讨白细胞介素-18(IL-18)对早期心肌缺血(EMI)和心肌梗塞(MI)死后诊断的法医学意义。方法筛选出34例心脏标本,分EMI(11例)、MI(10例)和对照(13例)组,检测心肌IL-18免疫组化染色(SP法)反应,并经全自动图像分析系统分析得到平均光密度值(AOD),所得数据进行统计学分析。结果对照组心肌IL-18免疫组化染色呈阴性,EMI组心肌呈阳性,MI组心肌呈强阳性;其AOD值分别是0.1193±0.0649、0.3827±0.0868、0.4792±0.0884,3组之间差异均具有显著性意义(P<0.05)。结论作为判断EMI和MI的指标之一,IL-18对EMI和MI的死后诊断具有法医学意义。Objective To investigate applicability of interleukin-18 （IL-18） to diagnosing of early myocardial ischemia （EMI） and myocardial infarction （MI） in postmortem specimens. Methods Myocardial samples collected from 34 atuopsied cases were employed in the experiment, which were divided into 3 groups, EMI（ 11 cases）, MI（ 10 cases）, and control （13 cases）. Immunoreactivity of IL-18 detected by immunohistochemical staining （S-P method）, and average optical density （AOD） were analyzed by automatic image analysis technique. The data were processed statistically. Results Weak or negative immunoreactivity of IL-18 was noted in the normal myocardial spcimens （NM） , positive in the EMI and significantly positive in the MI spcimens. AOD of immunostaining was measured as 0.1193±0. 0649, 0. 3827 ±0.0868 and 0.4792 ± 0.0884, respectively. There were significant difference between two among the three groups （P 〈0.05）. Conclusion It is indicated that IL-18 can be served as an predictor of EMI and MI, which may provide an method for postmortem diagnosis of EMI and MI.

关 键 词：法医病理学 白细胞介素-18(IL-18) 早期心肌缺血(EMI) 心肌梗死(MI) 

分 类 号：D919[医药卫生—法医学]