蜈蚣提取物诱导SiHa细胞凋亡及其作用机制的研究  

Effects of Centipede Extracts on Induction of Apoptosis and its Mechanism in SiHa Cell

作　　者：周永芹[1] 韩莉[1] 刘朝奇[2] 韩钰[2] 

机构地区：[1]三峡大学医学院免疫学重点学科,宜昌443000 [2]三峡大学分子生物学研究所

出　　处：《实用医学进修杂志》2007年第3期150-155,共6页Journal of Practical Training of Medicine

基　　金：湖北省教育厅2002年重点项目（2002A530011）

摘　　要：目的：研究蜈蚣提取物对宫颈癌SiHa细胞生长的抑制及其作用机制。方法：观察不同浓度的不同蜈蚣提取物对宫颈癌SiHa细胞的生长抑制情况：运用体外细胞培养技术,以不同浓度的蜈蚣提取物和阳性对照药顺铂作用于培养的SiHa细胞48h,采用MTT法测定药物对细胞生长的影响,以流式细胞术观察DNA含量和凋亡的变化情况,DNA片断化分析凋亡特有DNA梯形格局和Western Blot测定bax、Caspase3蛋白水平的表达。结果：SiHa细胞用8、16、32mg/ml的乙醚、乙醇蜈蚣提取物处理48h后,细胞生长显著受抑;DNA周期改变明显,亚G1峰与对照组有显著性差异;DNA-Ladder显著;Western Blot提示相关凋亡蛋白bax、Caspase3表达并显示活性,凋亡率增高明显。结论：中药蜈蚣乙醚、乙醇提取物在体外对宫颈癌SiHa细胞的生长有明显地抑制作用,其机制与改变细胞DNA周期,促进凋亡有关。Objective： To study the effects and mechanism of centipede extracts on proliferation of cervical cancer cell line SiHa. Methods： The growth of SiHa cell in vitro was observed with different concentration extracts by MTT ; DNA cycle and apoptosis detected by flow cytometry; DNA fragmentation assay done and bax and caspase 3 checked by western blotting. Results： The survival rate was decreased, the content of DNA in each stage cells and sub - G1 changed significantly than negative control, DNA ladder observed and bax, caspase 3 activated after SiHa cell treated with centipede components extracted with aether or ethanol （8,16 32 mg/ml）for 48 h. Conclusions： Centipede extracts could inhibit the growth of SiHa cell which is related to inhibitory DNA synthesis and enhance apoptosis in vitro.

关 键 词：蜈蚣提取物 SIHA细胞 细胞周期 DNA片断 凋亡 

分 类 号：R285.5[医药卫生—中药学]